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PMID: 8889521 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The dynein gene family in Chlamydomonas reinhardtii.

Genetics ·Vol. 144 ·No. 2 ·1996-10-00 ·Pages 569-85

Porter ME, Knott JA, Myster SH, Farlow SJ

Abstract

To correlate dynein heavy chain (Dhc) genes with flagellar mutations and gain insight into the function of specific dynein isoforms, we placed eight members of the Dhc gene family on the genetic map of Chlamydomonas. Using a PCR-based strategy, we cloned 11 Dhc genes from Chlamydomonas. Comparisons with other Dhc genes indicate that two clones correspond to genes encoding the alpha and beta heavy chains of the outer dynein arm. Alignment of the predicted amino acid sequences spanning the nucleotide binding site indicates that the remaining nine clones can be subdivided into three groups that are likely to include representatives of the inner-arm Dhc isoforms. Gene-specific probes reveal that each clone represents a single-copy gene that is expressed as a transcript of the appropriate size (> 13 kb) sufficient to encode a high molecular weight Dhc polypeptide. The expression of all nine genes is upregulated in response to deflagellation, suggesting a role in axoneme assembly or motility. Restriction fragment length polymorphisms between divergent C. reinhardtii strains have been used to place each Dhc gene on the genetic map of Chlamydomonas. These studies lay the groundwork for correlating defects in different Dhc genes with specific flagellar mutations.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chlamydomonas reinhardtii/enzymology,genetics Chromosome Mapping Cloning, Molecular DNA, Protozoan Dyneins/genetics Gene Expression Genes, Protozoan Molecular Sequence Data Polymorphism, Restriction Fragment Length Protozoan Proteins/genetics Sequence Homology, Amino Acid
Chemicals
DNA, Protozoan Protozoan Proteins Dyneins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Porter M E
Department of Cell Biology and Neuroanatomy, University of Minnesota Medical School, Minneapolis 55455, USA. mary-p@biosci.cbs.umn.edu
Knott J A
Myster S H
Farlow S J
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/144.2.569
Email
mary-p@biosci.cbs.umn.edu
Published
1996-10-00
Pages
569-85
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1207551
Subset
IM
Databases
GENBANK
L23195, L23196, L23197, L26049, U02963, U03969, U03970, U03971, U03972, U03973, U03974, U03975, U03976, U03977, U03978, U03979, U03980, U15303, U61364, U61365, U61366, U61367, U61368, U61369, U61370, U61371, U61372, X59603, Z21941
External Links
PubMed source DOI: 10.1093/genetics/144.2.569 PMC Full Text View journal details
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