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PMID: 1672692 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular studies of linkage group XIX of Chlamydomonas reinhardtii: evidence against a basal body location.

The Journal of cell biology ·Vol. 113 ·No. 2 ·1991-04-00 ·Pages 339-46

Johnson DE, Dutcher SK

Abstract

Linkage group XIX (also known as the UNI linkage group) in the green alga, Chlamydomonas reinhardtii, exhibits a number of unusual properties that have lead to the suggestion that it represents a basal body-associated chromosome. To begin a molecular analysis of this linkage group, we have identified DNA sequences from it and used them to determine the copy number of linkage group XIX within the cell. We find that linkage group XIX is present in the same copy number per cell as nuclear linkage groups in both haploid and diploid strains. We also find that the copy number of linkage group XIX is unchanged in mutants lacking basal bodies. We conclude that there is no convincing evidence that linkage group XIX localizes to the basal bodies of Chlamydomonas reinhardtii cells.

MeSH Terms
Base Sequence Blotting, Southern Centrioles Chlamydomonas/genetics,ultrastructure Chromosome Mapping Cloning, Molecular DNA/isolation & purification Genetic Linkage Haploidy Nucleic Acid Hybridization Polymorphism, Restriction Fragment Length
Chemicals
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Johnson D E
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347.
Dutcher S K
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.113.2.339
Published
1991-04-00
Pages
339-46
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2288942
Subset
IM
Grants
NIGMS NIH HHS · R01 GM032843 · United States
NIGMS NIH HHS · GM32843 · United States
NIGMS NIH HHS · GM5T32M07135 · United States
External Links
PubMed source DOI: 10.1083/jcb.113.2.339 PMC Full Text View journal details
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