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PMID: 3855249 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The two alpha-tubulin genes of Chlamydomonas reinhardi code for slightly different proteins.

Molecular and cellular biology ·Vol. 5 ·No. 9 ·1985-09-00 ·Pages 2389-98

Silflow CD, Chisholm RL, Conner TW, Ranum LP

Abstract

Full-length cDNA clones corresponding to the transcripts of the two alpha-tubulin genes in Chlamydomonas reinhardi were isolated. DNA sequence analysis of the cDNA clones and cloned gene fragments showed that each gene contains 1,356 base pairs of coding sequence, predicting alpha-tubulin products of 451 amino acids. Of the 27 nucleotide differences between the two genes, only two result in predicted amino acid differences between the two gene products. In the more divergent alpha 2 gene, a leucine replaces an arginine at amino acid 308, and a valine replaces a glycine at amino acid 366. The results predicted that two alpha-tubulin proteins with different net charges are produced as primary gene products. The predicted amino acid sequences are 86 and 70% homologous with alpha-tubulins from rat brain and Schizosaccharomyces pombe, respectively. Each gene had two intervening sequences, located at identical positions. Portions of an intervening sequence highly conserved between the two beta-tubulin genes are also found in the second intervening sequence of each of the alpha genes. These results, together with our earlier report of the beta-tubulin sequences in C. reinhardi, present a picture of the total complement of genetic information for tubulin in this organism.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chlamydomonas/genetics DNA/genetics DNA, Recombinant/analysis Genes Rats/genetics Saccharomyces/genetics Sequence Homology, Nucleic Acid Tubulin/genetics
Chemicals
DNA, Recombinant Tubulin DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Silflow C D
Chisholm R L
Conner T W
Ranum L P
References (42)
42 references, click to expand
  1. Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas.
    J Cell Biol. 1967 Jun;33(3):543-71 PMID: 5341020
  2. mRNA abundance changes during flagellar regeneration in Chlamydomonas reinhardtii.
    Mol Cell Biol. 1984 Mar;4(3):424-34 PMID: 6546968
  3. Fine structure of cell division in Chlamydomonas reinhardi. Basal bodies and microtubules.
    J Cell Biol. 1968 Aug;38(2):403-25 PMID: 5664210
  4. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  5. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.
    J Biol Chem. 1978 Apr 10;253(7):2483-95 PMID: 75887
  6. Primary structural differences among tubulin subunits from flagella, cilia, and the cytoplasm.
    Biochemistry. 1978 Jul 11;17(14):2882-91 PMID: 567486
  7. Increased levels of mRNAs for tubulin and other flagellar proteins after amputation or shortening of Chlamydomonas flagella.
    Cell. 1980 Jun;20(2):469-77 PMID: 6156007
  8. A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes.
    Gene. 1980 Aug;10(3):249-59 PMID: 6254843
  9. Nucleotide and corresponding amino acid sequences encoded by alpha and beta tubulin mRNAs.
    Nature. 1981 Feb 19;289(5799):650-5 PMID: 7464932
  10. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  11. Multiple alpha- and beta-tubulin genes in Chlamydomonas and regulation of tubulin mRNA levels after deflagellation.
    Cell. 1981 Apr;24(1):81-8 PMID: 6263492
  12. A beta-tubulin mutation in Aspergillus nidulans that blocks microtubule function without blocking assembly.
    Cell. 1981 Jun;24(3):837-45 PMID: 7018696
  13. Complete amino acid sequence of alpha-tubulin from porcine brain.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2757-61 PMID: 7019911
  14. Comparison of proteolytic cleavage patterns of alpha-tubulins and beta-tubulins from taxonomically distant species.
    J Mol Biol. 1981 Jun 15;149(1):95-107 PMID: 7031267
  15. Nucleotide sequence and evolution of a mammalian alpha-tubulin messenger RNA.
    J Mol Biol. 1981 Sep 5;151(1):101-20 PMID: 7328649
  16. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  17. Coordinate regulation of the four tubulin genes of Chlamydomonas reinhardi.
    Nucleic Acids Res. 1982 Feb 25;10(4):1295-310 PMID: 6280139
  18. Post-translational modification of tubulin dependent on organelle assembly.
    Nature. 1982 Jun 10;297(5866):516-8 PMID: 7088133
  19. Expression of flagellar protein genes during flagellar regeneration in Chlamydomonas.
    Cold Spring Harb Symp Quant Biol. 1982;46 Pt 1:157-69 PMID: 6179692
  20. Cell cycle stage-specific accumulation of mRNAs encoding tubulin and other polypeptides in Chlamydomonas.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5577-81 PMID: 6182565
  21. The testis-specific beta-tubulin subunit in Drosophila melanogaster has multiple functions in spermatogenesis.
    Cell. 1982 Dec;31(3 Pt 2):655-70 PMID: 6819086
  22. Multiple forms of tubulin in Polytomella and Chlamydomonas: evidence for a precursor of flagellar alpha-tubulin.
    J Cell Biol. 1983 Apr;96(4):1056-63 PMID: 6833391
  23. Apple II computer software for DNA and protein sequence data.
    DNA. 1983;2(1):31-5 PMID: 6687867
  24. Brain and erythrocyte microtubules from chicken contain different beta-tubulin polypeptides.
    J Biol Chem. 1983 Jun 25;258(12):7870-5 PMID: 6345531
  25. Chlamydomonas alpha-tubulin is posttranslationally modified in the flagella during flagellar assembly.
    J Cell Biol. 1983 Jul;97(1):258-63 PMID: 6863393
  26. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  27. Identification of the pleiotropic cell division cycle gene NDA2 as one of two different alpha-tubulin genes in Schizosaccharomyces pombe.
    Cell. 1984 May;37(1):233-42 PMID: 6327053
  28. Repeated consensus sequence and pseudopromoters in the four coordinately regulated tubulin genes of Chlamydomonas reinhardi.
    Mol Cell Biol. 1984 Jun;4(6):1115-24 PMID: 6738533
  29. Flagellar regeneration of the trypanosome Crithidia fasciculata involves post-translational modification of cytoplasmic alpha tubulin.
    Mol Cell Biol. 1984 Jun;4(6):1182-5 PMID: 6204192
  30. Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo.
    Cell. 1983 May;33(1):211-9 PMID: 6380751
  31. Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro.
    Cell. 1984 Aug;38(1):317-31 PMID: 6088074
  32. Distinct populations of microtubules: tyrosinated and nontyrosinated alpha tubulin are distributed differently in vivo.
    Cell. 1984 Oct;38(3):779-89 PMID: 6386177
  33. Sequence of a highly divergent beta tubulin gene reveals regional heterogeneity in the beta tubulin polypeptide.
    J Cell Biol. 1984 Nov;99(5):1754-60 PMID: 6490718
  34. Tubulin proteins and RNA during the myxamoeba-flagellate transformation of Physarum polycephalum.
    Mol Cell Biol. 1984 Sep;4(9):1706-11 PMID: 6208478
  35. Reversal of the posttranslational modification on Chlamydomonas flagellar alpha-tubulin occurs during flagellar resorption.
    J Cell Biol. 1985 Feb;100(2):457-62 PMID: 3968171
  36. The two beta-tubulin genes of Chlamydomonas reinhardtii code for identical proteins.
    Mol Cell Biol. 1984 Dec;4(12):2686-96 PMID: 6098820
  37. Chlamydomonas alpha-tubulin is posttranslationally modified by acetylation on the epsilon-amino group of a lysine.
    Biochemistry. 1985 Jan 15;24(2):473-8 PMID: 3919761
  38. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.
    Plasmid. 1985 Jan;13(1):31-40 PMID: 3991809
  39. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  40. Developmental rearrangements associated with a single type of expressed alpha-tubulin gene in Tetrahymena.
    Cell. 1984 Feb;36(2):441-5 PMID: 6319024
  41. Immunocytochemical demonstration of alpha-tubulin modification during axonal maturation in the cerebellar cortex.
    J Cell Biol. 1984 Jan;98(1):347-51 PMID: 6707095
  42. Replication of chloroplast DNA in Chlamydomonas reinhardi during vegetative cell cycle: its mode and regulation.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1506-13 PMID: 5231756
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.9.2389-2398.1985
Published
1985-09-00
Pages
2389-98
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366966
Subset
IM
Grants
PHS HHS · MG 31159 · United States
Databases
GENBANK
M11447, M11448
External Links
PubMed source DOI: 10.1128/mcb.5.9.2389-2398.1985 PMC Full Text View journal details
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