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PMID: 8858178 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Intracellular signaling is changed after clustering of the neural cell adhesion molecules axonin-1 and NgCAM during neurite fasciculation.

The Journal of cell biology ·Vol. 135 ·No. 1 ·1996-10-00 ·Pages 253-67

Kunz S, Ziegler U, Kunz B, Sonderegger P

Abstract

Neural cell adhesion molecules of the immunoglobulin/fibronectin type III family on axons have been implicated in promotion of neurite outgrowth, fasciculation, and the mediation of specific cell adhesion. The present study demonstrates that two of these molecules on dorsal root ganglion neurons are associated with distinct protein kinases, axonin-1 with the src-related nonreceptor tyrosine kinase fyn and NgCAM with a casein kinase II-related activity and a serine/ threonine kinase related to S6 kinase. When neurites grew without contacts involving axonin-1 and NgCAM, strong fyn kinase activity was associated with axonin-1, whereas the NgCAM-associated kinase activities were low. Clustering of axonin-1 with NgCAM induced by the formation of cell-cell contacts correlated with a reduction of the axonin-1-associated fyn activity and an increased phosphorylation of NgCAM by the associated casein kinase II-related activity. Thus, axonin-1 and NgCAM trigger distinctive intracellular signals during in vitro differentiation depending on their state of association.

MeSH Terms
Animals Casein Kinase II Cell Adhesion Molecules, Neuron-Glia/chemistry,metabolism Cell Adhesion Molecules, Neuronal/chemistry,metabolism Cell Communication/physiology Cell Differentiation Cells, Cultured Chick Embryo Contactin 2 Enzyme Inhibitors/pharmacology Ganglia, Spinal/physiology Genistein Heparin/pharmacology Isoflavones/pharmacology Molecular Weight Neurites/physiology Oligonucleotides, Antisense Phosphorylation Phosphotyrosine/analysis Precipitin Tests Protein Serine-Threonine Kinases/metabolism Protein-Tyrosine Kinases/antagonists & inhibitors Proto-Oncogene Proteins/metabolism Proto-Oncogene Proteins c-fyn Ribosomal Protein S6 Kinases Signal Transduction/physiology
Chemicals
Cell Adhesion Molecules, Neuron-Glia Cell Adhesion Molecules, Neuronal Contactin 2 Enzyme Inhibitors Isoflavones Oligonucleotides, Antisense Proto-Oncogene Proteins Phosphotyrosine Heparin Genistein Protein-Tyrosine Kinases Proto-Oncogene Proteins c-fyn Casein Kinase II Protein Serine-Threonine Kinases Ribosomal Protein S6 Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kunz S
Institute of Biochemistry, University of Zurich, Switzerland.
Ziegler U
Kunz B
Sonderegger P
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.135.1.253
Published
1996-10-00
Pages
253-67
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2121033
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.135.1.253 PMC Full Text View journal details
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