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PMID: 3025222 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Distinct molecular interactions mediate neuronal process outgrowth on non-neuronal cell surfaces and extracellular matrices.

The Journal of cell biology ·Vol. 103 ·No. 6 Pt 2 ·1986-12-00 ·Pages 2659-72

Tomaselli KJ, Reichardt LF, Bixby JL

Abstract

We have compared neurite outgrowth on extracellular matrix (ECM) constituents to outgrowth on glial and muscle cell surfaces. Embryonic chick ciliary ganglion (CG) neurons regenerate neurites rapidly on surfaces coated with laminin (LN), fibronectin (FN), conditioned media (CM) from several non-neuronal cell types that secrete LN, and on intact extracellular matrices. Neurite outgrowth on all of these substrates is blocked by two monoclonal antibodies, CSAT and JG22, that prevent the adhesion of many cells, including neurons, to the ECM constituents LN, FN, and collagen. Neurite outgrowth is inhibited even on mixed LN/poly-D-lysine substrates where neuronal attachment is independent of LN. Therefore, neuronal process outgrowth on extracellular matrices requires the function of neuronal cell surface molecules recognized by these antibodies. The surfaces of cultured astrocytes, Schwann cells, and skeletal myotubes also promote rapid process outgrowth from CG neurons. Neurite outgrowth on these surfaces, though, is not prevented by CSAT or JG22 antibodies. In addition, antibodies to a LN/proteoglycan complex that block neurite outgrowth on several LN-containing CM factors and on an ECM extract failed to inhibit cell surface-stimulated neurite outgrowth. After extraction with a nonionic detergent, Schwann cells and myotubes continue to support rapid neurite outgrowth. However, the activity associated with the detergent insoluble residue is blocked by CSAT and JG22 antibodies. Detergent extraction of astrocytes, in contrast, removes all neurite-promoting activity. These results provide evidence for at least two types of neuronal interactions with cells that promote neurite outgrowth. One involves adhesive proteins present in the ECM and ECM receptors on neurons. The second is mediated through detergent-extractable macromolecules present on non-neuronal cell surfaces and different, uncharacterized receptor(s) on neurons. Schwann cells and skeletal myotubes appear to promote neurite outgrowth by both mechanisms.

MeSH Terms
Animals Antibodies, Monoclonal Cell Adhesion Cell Differentiation Cells, Cultured Chick Embryo Extracellular Matrix/physiology Fibronectins/physiology Ganglia/cytology Laminin/physiology Muscles/embryology Neurons/cytology Receptors, Cell Surface/immunology,physiology Schwann Cells/physiology
Chemicals
Antibodies, Monoclonal Fibronectins Laminin Receptors, Cell Surface
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tomaselli K J
Reichardt L F
Bixby J L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.103.6.2659
Published
1986-12-00
Pages
2659-72
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114572
Subset
IM
Grants
NINDS NIH HHS · NS 19090 · United States
External Links
PubMed source DOI: 10.1083/jcb.103.6.2659 PMC Full Text View journal details
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