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PMID: 7682713 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Thy-1 glycoprotein and src-like protein-tyrosine kinase p53/p56lyn are associated in large detergent-resistant complexes in rat basophilic leukemia cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 8 ·1993-04-15 ·Pages 3611-5

Dráberová L, Dráber P

Abstract

Thy-1 is a surface glycoprotein that is attached to the plasma membrane by a glycosyl-phosphatidyl-inositol anchor. Crosslinking of Thy-1 in rat mast cells and basophilic leukemia cells (RBL-2H3) induces cell activation including histamine release and tyrosine phosphorylation of several proteins. Here we show that glycosyl-phosphatidylinositol-linked Thy-1 forms noncovalent complexes with src-related protein-tyrosine kinase p53/p56lyn and other protein-tyrosine kinases and/or their substrates. These complexes are resistant to solubilization by a nonionic detergent, sedimentable at 200,000 x g, and very large ( > 10 MDa) as determined by gel chromatography. Activation of RBL-2H3 cells by crosslinking of the high-affinity IgE receptors resulted in decreased recovery of the complexes. The combined data indicate the existence of large detergent-resistant domains in the surface membrane of mast cells that may play an important role in their activation.

MeSH Terms

Animals Antibodies, Monoclonal Antigens, Surface/isolation & purification,metabolism CSK Tyrosine-Protein Kinase Chromatography, Gel Electrophoresis, Polyacrylamide Gel Immunoblotting Leukemia, Basophilic, Acute Macromolecular Substances Membrane Glycoproteins/isolation & purification,metabolism Molecular Weight Phosphatidylinositol Diacylglycerol-Lyase Phosphoric Diester Hydrolases Protein-Tyrosine Kinases/isolation & purification,metabolism Proto-Oncogene Proteins pp60(c-src)/isolation & purification,metabolism Rats Receptors, IgE/metabolism Thy-1 Antigens Tumor Cells, Cultured src-Family Kinases

Chemicals

Antibodies, Monoclonal Antigens, Surface Macromolecular Substances Membrane Glycoproteins Receptors, IgE Thy-1 Antigens Protein-Tyrosine Kinases CSK Tyrosine-Protein Kinase Proto-Oncogene Proteins pp60(c-src) lyn protein-tyrosine kinase src-Family Kinases Phosphoric Diester Hydrolases Phosphatidylinositol Diacylglycerol-Lyase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Dráberová L

Department of Mammalian Gene Expression, Czechoslovak Academy of Sciences, Prague.

Dráber P

References (29)

29 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.90.8.3611

Published

1993-04-15

Pages

3611-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC46351

Subset

External Links

PubMed source DOI: 10.1073/pnas.90.8.3611 PMC Full Text View journal details

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Thy-1 glycoprotein and src-like protein-tyrosine kinase p53/p56lyn are associated in large detergent-resistant complexes in rat basophilic leukemia cells.

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