Home LiteratureArticle Details
PMID: 8815111 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Assessment of genetic markers for species differentiation within the Mycobacterium tuberculosis complex.

Journal of clinical microbiology ·Vol. 34 ·No. 4 ·1996-04-00 ·Pages 933-8

Liébana E, Aranaz A, Francis B, Cousins D

Abstract

It is important to correctly identify species within the Mycobacterium tuberculosis complex because of the zoonotic implications of bovine tuberculosis, especially in developing countries. We assessed the use of various genetic markers for species-specific identification of mycobacteria from the M. tuberculosis complex. A multiplex PCR designed for detection of the mtp40 and IS1081 elements was optimized and evaluated in 339 mycobacterial strains from different animal and geographic origins. The host range of the IS6110, MPB70, and 16S rRNA genes was also studied by PCR in all the strains. Finally, the usefulness of the genetic markers was compared by an immunoperoxidase test for specific identification of Mycobacterium bovis strains. The mtp40 sequence was detected in 87 of the 91 strains of M. tuberculosis and in 9 of the 11 Mycobacterium africanum strains but not in any of the M. bovis or Mycobacterium microti strains, indicating that the mtp40 element was also found in all of the M. tuberculosis complex strains isolated from seals. This organism is considered to be a true seal pathogen, but its origin is essentially unknown. The finding of the mtp40 element in the strains from seals suggests a closer relationship of these strains with a human origin than to an animal origin. The mtp40 element was not found in any other mycobacterial species included in the study. As a result of this study, we suggest that biochemical tests or alternate genetic markers are still needed to differentiate M. tuberculosis from M. africanum when these species coexist as causative agents of tuberculosis. The immunoperoxidase test worked well for the identification of M. bovis strains. We also report, for the first time, PCR amplification of the repetitive element IS6110 in an isolate of Mycobacterium ulcerans and an isolate of Mycobacterium gilvum, which emphasizes the need for further investigation of the host range of this sequence.

MeSH Terms
Animals Base Sequence Cattle DNA Primers/genetics DNA Transposable Elements DNA, Bacterial/genetics Evaluation Studies as Topic Genes, Bacterial Genetic Markers Humans Immunoenzyme Techniques Molecular Sequence Data Mycobacterium/classification,genetics,pathogenicity Mycobacterium tuberculosis/classification,genetics,pathogenicity Polymerase Chain Reaction/methods RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Species Specificity Tuberculosis, Bovine/microbiology Zoonoses/microbiology
Chemicals
DNA Primers DNA Transposable Elements DNA, Bacterial Genetic Markers RNA, Bacterial RNA, Ribosomal, 16S
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Liébana E
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
Aranaz A
Francis B
Cousins D
References (39)
39 references, click to expand
  1. Characterization of Mycobacterium tuberculosis strains from Vietnamese patients by Southern blot hybridization.
    J Clin Microbiol. 1993 Jun;31(6):1615-8 PMID: 8100244
  2. Use of a repetitive element isolated from Mycobacterium tuberculosis in hybridization studies with Mycobacterium bovis: a new tool for epidemiological studies of bovine tuberculosis.
    Vet Microbiol. 1993 Oct;37(1-2):1-17 PMID: 7905219
  3. HIV/AIDS and its implications for the control of animal tuberculosis.
    Br Vet J. 1993 Sep-Oct;149(5):405-17 PMID: 8298954
  4. Extensive DNA sequence conservation throughout the Mycobacterium tuberculosis complex.
    J Clin Microbiol. 1994 Jul;32(7):1639-43 PMID: 7929750
  5. Tuberculosis in imported hyrax (Procavia capensis) caused by an unusual variant belonging to the Mycobacterium tuberculosis complex.
    Vet Microbiol. 1994 Nov;42(2-3):135-45 PMID: 7886928
  6. Demonstration of homology between IS6110 of Mycobacterium tuberculosis and DNAs of other Mycobacterium spp.?
    J Clin Microbiol. 1995 Sep;33(9):2290-3 PMID: 7494016
  7. A multiplex polymerase chain reaction for distinguishing Mycobacterium tuberculosis from Mycobacterium tuberculosis complex.
    Mol Cell Probes. 1995 Oct;9(5):291-5 PMID: 8569767
  8. IS6110, an IS-like element of Mycobacterium tuberculosis complex.
    Nucleic Acids Res. 1990 Jan 11;18(1):188 PMID: 2155396
  9. Advantages of a new agar medium in the primary isolation of Mycobacterium bovis.
    Vet Microbiol. 1989 May;20(1):89-95 PMID: 2669322
  10. Production and characterization of monoclonal antibodies specific for Mycobacterium bovis.
    J Gen Microbiol. 1988 Sep;134(9):2599-604 PMID: 2474629
  11. Incidence and nature of human tuberculosis due to bovine tubercle bacilli in South-East England: 1977-1987.
    Epidemiol Infect. 1988 Oct;101(2):225-9 PMID: 3053216
  12. Nontuberculous mycobacteria and Mycobacterium bovis as a cause of human disease in Argentina.
    Trop Geogr Med. 1987 Jul;39(3):222-7 PMID: 3124310
  13. Pulmonary tuberculosis due to Mycobacterium bovis.
    Thorax. 1986 Sep;41(9):685-7 PMID: 3538491
  14. Isolation of Mycobacterium africanum from monkeys.
    Tubercle. 1980 Jun;61(2):101-4 PMID: 6776671
  15. Mycobacterium microti infection in a zoo-llama: Lama vicugna (Molina).
    Acta Zool Pathol Antverp. 1970 Sep;51:17-24 PMID: 5527701
  16. Bovine tuberculosis in humans in Ontario. The epidemiologic features of 31 active cases occurring between 1964 and 1970.
    Am Rev Respir Dis. 1972 Oct;106(4):528-34 PMID: 4627829
  17. Observations on human infection with Mycobacterium bovis.
    Tubercle. 1969 Mar;50(1):14-21 PMID: 5813787
  18. Pulmonary bovine tuberculosis in an owner and in his dairy herd.
    Cornell Vet. 1968 Jan;58(1):81-7 PMID: 5690333
  19. Mycobacterium microti infection in a cat and some pigs.
    Antonie Van Leeuwenhoek. 1967;33(2):209-12 PMID: 5298436
  20. A fatal case of infection due to Mycobacterium bovis.
    Am Rev Respir Dis. 1966 May;93(5):804-10 PMID: 5327835
  21. Epitope mapping of the Mycobacterium bovis secretory protein MPB70 using overlapping peptide analysis.
    J Gen Microbiol. 1990 Feb;136(2):265-72 PMID: 1691265
  22. Tuberculosis in captive seals: bacteriological studies on an isolate belonging to the Mycobacterium tuberculosis complex.
    Res Vet Sci. 1990 Mar;48(2):196-200 PMID: 2110376
  23. Rapid confirmatory identification of Mycobacterium bovis using a dot blotting immunodetection technique.
    Vet Microbiol. 1990 May;22(4):335-40 PMID: 2114051
  24. DNA fingerprinting of Mycobacterium bovis strains by restriction fragment analysis and hybridization with insertion elements IS1081 and IS6110.
    J Clin Microbiol. 1993 May;31(5):1143-7 PMID: 8099083
  25. Tuberculosis in wild seals and characterisation of the seal bacillus.
    Aust Vet J. 1993 Mar;70(3):92-7 PMID: 8097394
  26. Mycobacterium bovis infections in San Diego: a clinicoepidemiologic study of 73 patients and a historical review of a forgotten pathogen.
    Medicine (Baltimore). 1993 Jan;72(1):11-37 PMID: 8426535
  27. Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube.
    PCR Methods Appl. 1992 May;1(4):269-73 PMID: 1282431
  28. Zoonotic tuberculosis in Latin America.
    J Clin Microbiol. 1992 Dec;30(12):3299-300 PMID: 1452723
  29. Evaluation of nonradioactive DNA probes for identification of mycobacteria.
    J Clin Microbiol. 1992 Sep;30(9):2476-8 PMID: 1401020
  30. Identification of an insertion sequence, IS1081, in Mycobacterium bovis.
    FEMS Microbiol Lett. 1991 Sep 15;67(1):11-5 PMID: 1663885
  31. Evaluation of acridinium-ester-labeled DNA probes for identification of Mycobacterium tuberculosis and Mycobacterium avium-Mycobacterium intracellulare complex in culture.
    J Clin Microbiol. 1991 Nov;29(11):2473-6 PMID: 1723071
  32. Amplification of a species-specific DNA fragment of Mycobacterium tuberculosis and its possible use in diagnosis.
    J Clin Microbiol. 1991 Oct;29(10):2163-8 PMID: 1939567
  33. Isolation, characterization, and molecular cloning of a specific Mycobacterium tuberculosis antigen gene: identification of a species-specific sequence.
    Infect Immun. 1991 Oct;59(10):3411-7 PMID: 1909999
  34. Use of DNA amplification for the rapid identification of Mycobacterium bovis.
    Vet Microbiol. 1991 Apr;27(2):187-95 PMID: 2063549
  35. Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium.
    Int J Syst Bacteriol. 1990 Oct;40(4):323-30 PMID: 2275850
  36. Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis.
    J Clin Microbiol. 1990 Sep;28(9):2051-8 PMID: 1977765
  37. Detection and identification of mycobacteria by amplification of rRNA.
    J Clin Microbiol. 1990 Aug;28(8):1751-9 PMID: 2203812
  38. Comparison of various repetitive DNA elements as genetic markers for strain differentiation and epidemiology of Mycobacterium tuberculosis.
    J Clin Microbiol. 1993 Aug;31(8):1987-95 PMID: 7690367
  39. A nosocomial outbreak of multidrug-resistant Mycobacterium bovis among HIV-infected patients. A case-control study.
    AIDS. 1993 Nov;7(11):1453-60 PMID: 8280411
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.34.4.933-938.1996
Published
1996-04-00
Pages
933-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC228920
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.34.4.933-938.1996 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2