Evaluation of nonradioactive DNA probes for identification of mycobacteria.

Journal of clinical microbiology ·Vol. 30 ·No. 9 ·1992-09-00 ·Pages 2476-8

Lebrun L, Espinasse F, Poveda JD, Vincent-Levy-Frebault V

Abstract

Commercial chemiluminescent DNA probes (Accuprobe; Gen-Probe, San Diego, Calif.) for the identification of Mycobacterium tuberculosis (MTB) complex, M. avium complex (MAC), M. gordonae, and M. kansasii were evaluated with 134 clinical isolates. These included 36 MTB complex, 40 MAC, 27 M. gordonae, 9 M. kansasii, and 22 Mycobacterium spp. The specificity was 100% for the four probes. The sensitivity was 100% for the MTB complex and M. gordonae probes and 95.2% for the MAC probe. Five of the nine M. kansasii isolates tested were not detected with the probe.

MeSH Terms

DNA Probes Evaluation Studies as Topic False Negative Reactions Luminescent Measurements Mycobacterium/classification,genetics,isolation & purification Sensitivity and Specificity

Chemicals

DNA Probes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Lebrun L

Service de Microbiologie, Hôpital Antoine Béclère, Clamart, France.

Espinasse F

Poveda J D

Vincent-Levy-Frebault V

References (12)

12 references, click to expand

Evaluation of acridinium-ester-labeled DNA probes for identification of Mycobacterium tuberculosis and Mycobacterium avium-Mycobacterium intracellulare complex in culture.
J Clin Microbiol. 1991 Nov;29(11):2473-6 PMID: 1723071
Identification of Mycobacterium gordonae from culture by the Gen-Probe Rapid Diagnostic System: evaluation of 218 isolates and potential sources of false-negative results.
J Clin Microbiol. 1991 Sep;29(9):1850-4 PMID: 1774307
Disseminated mixed Mycobacterium simiae-Mycobacterium avium complex infection in acquired immunodeficiency syndrome.
J Infect Dis. 1991 Aug;164(2):432-3 PMID: 1856495
Identification of various serovar strains of Mycobacterium avium complex by using DNA probes specific for Mycobacterium avium and Mycobacterium intracellulare.
J Clin Microbiol. 1990 Aug;28(8):1694-7 PMID: 2203807
Gen-probe test should not be considered final in Mycobacterium tuberculosis identification.
J Clin Microbiol. 1989 Jan;27(1):229 PMID: 2492302
Rapid radiometric methods to detect and differentiate Mycobacterium tuberculosis/M. bovis from other mycobacterial species.
Am Rev Respir Dis. 1984 Oct;130(4):634-40 PMID: 6435489
Assay formats involving acridinium-ester-labeled DNA probes.
Clin Chem. 1989 Aug;35(8):1588-94 PMID: 2667804
Rapid identification of Mycobacterium avium complex in culture using DNA probes.
J Clin Microbiol. 1987 Aug;25(8):1442-5 PMID: 3114319
Evaluation of Gen-Probe DNA hybridization systems for the identification of Mycobacterium tuberculosis and Mycobacterium avium-intracellulare.
Diagn Microbiol Infect Dis. 1987 Oct;8(2):69-77 PMID: 3123122
Identification of mycobacteria from culture by using the Gen-Probe Rapid Diagnostic System for Mycobacterium avium complex and Mycobacterium tuberculosis complex.
J Clin Microbiol. 1988 Oct;26(10):2120-3 PMID: 3141459
Mycobacterium simiae and Mycobacterium avium-M. intracellulare mixed infection in acquired immune deficiency syndrome.
J Clin Microbiol. 1987 Jan;25(1):154-7 PMID: 3793869
Direct identification of Mycobacterium tuberculosis, Mycobacterium avium, and Mycobacterium intracellulare from amplified primary cultures in BACTEC media using DNA probes.
J Clin Microbiol. 1989 Jul;27(7):1543-7 PMID: 2504770

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.30.9.2476-2478.1992

Published

1992-09-00

Pages

2476-8

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC265528

Subset

External Links

PubMed source DOI: 10.1128/jcm.30.9.2476-2478.1992 PMC Full Text View journal details

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Evaluation of nonradioactive DNA probes for identification of mycobacteria.

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