Home LiteratureArticle Details
PMID: 2203812 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection and identification of mycobacteria by amplification of rRNA.

Journal of clinical microbiology ·Vol. 28 ·No. 8 ·1990-08-00 ·Pages 1751-9

Böddinghaus B, Rogall T, Flohr T, Blöcker H, Böttger EC

Abstract

Oligonucleotides specific at a genus, group, or species level were defined by a systematic comparison of small-subunit rRNA sequences from Mycobacterium tuberculosis, M. bovis, M. africanum, M. bovis BCG, M. avium, M. kansasii, M. marinum, M. gastri, M. chelonae, M. smegmatis, M. terrae, M. nonchromogenicum, M. xenopi, M. malmoense, M. szulgai, M. scrofulaceum, M. fortuitum, M. gordonae, M. intracellulare, M. simiae, M. flavescens, M. paratuberculosis, M. sphagni, M. cookii, M. komossense, M. phlei, and M. farcinica. On the basis of the defined oligonucleotides, the polymerase chain reaction technique was explored to develop a sensitive taxon-specific detection system for mycobacteria. By using M. tuberculosis as a model system, fewer than 10 bacteria could be reliably detected by this kind of assay. These results suggest that amplification of rRNA sequences by the polymerase chain reaction may provide a highly sensitive and specific tool for the direct detection of microorganisms without the need for prior cultivation.

MeSH Terms
Base Sequence DNA, Bacterial/isolation & purification Gene Amplification Molecular Sequence Data Mycobacterium/classification,genetics Oligonucleotide Probes Polymerase Chain Reaction RNA, Ribosomal/biosynthesis RNA, Ribosomal, 16S/biosynthesis Sensitivity and Specificity Species Specificity Transcription, Genetic
Chemicals
DNA, Bacterial Oligonucleotide Probes RNA, Ribosomal RNA, Ribosomal, 16S
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Böddinghaus B
Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Federal Republic of Germany.
Rogall T
Flohr T
Blöcker H
Böttger E C
References (17)
17 references, click to expand
  1. Diagnosis of tuberculosis.
    Chest. 1979 Dec;76(6 Suppl):757-63 PMID: 116806
  2. Polymerase chain reaction for the detection of Mycobacterium leprae.
    J Gen Microbiol. 1989 Sep;135(9):2357-64 PMID: 2697743
  3. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses.
    Proc Natl Acad Sci U S A. 1985 Oct;82(20):6955-9 PMID: 2413450
  4. Bacterial evolution.
    Microbiol Rev. 1987 Jun;51(2):221-71 PMID: 2439888
  5. Oligonucleotide probes complementary to variable regions of ribosomal RNA discriminate between Mycoplasma species.
    J Gen Microbiol. 1987 Jul;133(7):1969-74 PMID: 2444670
  6. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools.
    Ann Inst Pasteur Microbiol. 1986 Sep-Oct;137B(2):165-75 PMID: 2446639
  7. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
    Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
  8. Compilation of small ribosomal subunit RNA sequences.
    Nucleic Acids Res. 1988;16 Suppl:r87-173 PMID: 2453029
  9. Nucleic acid hybridization studies of mycobactin-dependent mycobacteria.
    J Clin Microbiol. 1988 Jul;26(7):1309-12 PMID: 3410945
  10. Detection of enterotoxigenic Escherichia coli after polymerase chain reaction amplification with a thermostable DNA polymerase.
    J Clin Microbiol. 1989 Feb;27(2):261-5 PMID: 2644292
  11. AIDS-related mycobacterial disease.
    Springer Semin Immunopathol. 1988;10(4):375-91 PMID: 3065953
  12. Tenosynovitis of the hand caused by Mycobacterium terrae.
    Eur J Clin Microbiol Infect Dis. 1989 Aug;8(8):722-4 PMID: 2506040
  13. Detection and identification of mycobacteria by amplification of mycobacterial DNA.
    Mol Microbiol. 1989 Jul;3(7):843-9 PMID: 2507865
  14. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA.
    Nucleic Acids Res. 1989 Oct 11;17(19):7843-53 PMID: 2798131
  15. Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA.
    FEMS Microbiol Lett. 1989 Nov;53(1-2):171-6 PMID: 2482222
  16. Specific Neisseria gonorrhoeae DNA-probes derived from ribosomal RNA.
    J Gen Microbiol. 1989 Jun;135(6):1735-45 PMID: 2482333
  17. Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae.
    J Bacteriol. 1985 Mar;161(3):1093-102 PMID: 3882664
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.28.8.1751-1759.1990
Published
1990-08-00
Pages
1751-9
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC268042
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.28.8.1751-1759.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2