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PMID: 8656293 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of NGF and NT3 mRNAs in hippocampal interneurons innervated by the GABAergic septohippocampal pathway.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 16 ·No. 12 ·1996-06-15 ·Pages 3991-4004

Rocamora N, Pascual M, Acsàdy L, de Lecea L, Freund TF, Soriano E

Abstract

We used in situ hybridization for the detection of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin 3 (NT3) mRNAs combined with immunocytochemistry against the calcium-binding proteins parvalbumin (PARV), calbindin 28k (CALB), and calretinin (CALR) to determine the expression of neurotrophins in functionally distinct subsets of hippocampal interneurons. Most PARV-immunoreactive neurons in the hippocampus were NGF mRNA-positive (82%), which corresponds to 71% of NGF-positive neurons in the hippocampus proper and in the dentate gyrus (excluding granule cells). In contrast, only a subset of CALB- and CALR-immunoreactive interneurons (24% and 23%, respectively) displayed hybridization signals for NGF. Small subsets of PARV- and CALR-positive cells expressed NT3 mRNA, but we did not find hippocampal interneurons expressing BDNF mRNA. These results show that NGF and NT3 genes are differentially regulated in distinct subsets of GABAergic cells, and these interneurons are a major source of NGF production in the hippocampus. We also addressed whether hippocampal interneurons expressing neurotrophins were targets of the GABAergic septohippocampal pathway. We developed a triple-labeling method that combines anterograde tracing of this pathway by means of Phaseolus vulgaris leucoagglutinin injections, with in situ hybridization for the detection of neurotrophins, and immunocytochemistry for calcium-binding proteins. Virtually every PARV-positive neuron innervated by GABAergic septohippocampal baskets expressed NGF mRNA (86%), whereas 39-59% of CALR- and CALB-positive interneurons that were contacted by GABAergic septohippocampal axons showed NGF gene expression. A small subset of NT3 mRNA-expressing interneurons was also innervated by septohippocampal baskets. These findings show that the GABAergic septohippocampal pathway preferentially terminates on interneurons expressing NGF mRNA, suggesting that this neurotrophic factor might be involved in the specification of this connection and in its maintenance and normal function in the adult brain.

MeSH Terms
Afferent Pathways Animals Axons/chemistry Base Sequence Biomarkers Brain-Derived Neurotrophic Factor Cholinergic Fibers/chemistry,physiology Hippocampus/cytology Immunohistochemistry In Situ Hybridization Interneurons/cytology,physiology Molecular Sequence Data Nerve Growth Factors/analysis,genetics Nerve Tissue Proteins/analysis Neurotrophin 3 Phytohemagglutinins RNA, Messenger/physiology Rats Rats, Wistar Septal Nuclei/cytology gamma-Aminobutyric Acid/physiology
Chemicals
Biomarkers Brain-Derived Neurotrophic Factor Nerve Growth Factors Nerve Tissue Proteins Neurotrophin 3 Phytohemagglutinins RNA, Messenger leukoagglutinins, plants gamma-Aminobutyric Acid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rocamora N
Department of Animal and Plant Cell Biology, Faculty of Biology, University of Barcelona, Spain.
Pascual M
Acsàdy L
de Lecea L
Freund T F
Soriano E
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1996-06-15
Pages
3991-4004
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6578621
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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