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PMID: 2369898 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regional distribution of brain-derived neurotrophic factor mRNA in the adult mouse brain.

The EMBO journal ·Vol. 9 ·No. 8 ·1990-08-00 ·Pages 2459-64

Hofer M, Pagliusi SR, Hohn A, Leibrock J, Barde YA

Abstract

Brain-derived neurotrophic factor (BDNF) is a protein that allows the survival of specific neuronal populations. This study reports on the distribution of the BDNF mRNA in the adult mouse brain, where the BDNF gene is strongly expressed, using quantitative Northern blot analysis and in situ hybridization. All brain regions examined were found to contain substantial amounts of BDNF mRNA, the highest levels being found in the hippocampus followed by the cerebral cortex. In the hippocampus, which is also the site of highest nerve growth factor (NGF) gene expression in the central nervous system (CNS), there is approximately 50-fold more BDNF mRNA than NGF mRNA. In other brain regions, such as the granule cell layer of the cerebellum, the differences between the levels of BDNF and NGF mRNAs are even more pronounced. The BDNF mRNA was localized by in situ hybridization in hippocampal neurons (pyramidal and granule cells). These data suggest that BDNF may play an important role in the CNS for a wide variety of adult neurons.

MeSH Terms
Amino Acid Sequence Animals Autoradiography Base Sequence Brain/metabolism Brain-Derived Neurotrophic Factor Cloning, Molecular DNA/genetics Gene Library Mice Molecular Sequence Data Nerve Growth Factors/genetics Nerve Tissue Proteins/genetics Organ Specificity Phosphorus Radioisotopes RNA, Messenger/analysis,genetics
Chemicals
Brain-Derived Neurotrophic Factor Nerve Growth Factors Nerve Tissue Proteins Phosphorus Radioisotopes RNA, Messenger DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hofer M
Max-Planck Institute for Psychiatry, Department of Neurochemistry, Martinsried, FRG.
Pagliusi S R
Hohn A
Leibrock J
Barde Y A
References (33)
33 references, click to expand
  1. Molecular cloning and expression of brain-derived neurotrophic factor.
    Nature. 1989 Sep 14;341(6238):149-52 PMID: 2779653
  2. Growth characteristics of adrenergic nerves in the adult rat. Fluorescence histochemical and 3H-noradrenaline uptake studies using tissue transplantations to the anterior chamber of the eye.
    Acta Physiol Scand Suppl. 1970;348:1-112 PMID: 4320063
  3. Identification and characterization of a novel member of the nerve growth factor/brain-derived neurotrophic factor family.
    Nature. 1990 Mar 22;344(6264):339-41 PMID: 2314473
  4. Biochemical effects of antibodies against nerve growth factor on developing and differentiated sympathetic ganglia.
    Brain Res. 1978 Jun 9;148(1):264-8 PMID: 26453
  5. Development and regional expression of beta nerve growth factor messenger RNA and protein in the rat central nervous system.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):817-21 PMID: 3456170
  6. Physiology of nerve growth factor.
    Physiol Rev. 1980 Oct;60(4):1284-335 PMID: 6159658
  7. Human beta-nerve growth factor gene sequence highly homologous to that of mouse.
    Nature. 1983 Jun 30;303(5920):821-5 PMID: 6688123
  8. Studies on the expression of the beta nerve growth factor (NGF) gene in the central nervous system: level and regional distribution of NGF mRNA suggest that NGF functions as a trophic factor for several distinct populations of neurons.
    Proc Natl Acad Sci U S A. 1986 Apr;83(8):2714-8 PMID: 3458230
  9. Brain-derived neurotrophic factor prevents neuronal death in vivo.
    Nature. 1988 Jan 21;331(6153):261-2 PMID: 3336438
  10. Expression of the beta-nerve growth factor gene in hippocampal neurons.
    Science. 1988 Jun 3;240(4857):1339-41 PMID: 2897715
  11. PURIFICATION OF A NERVE-GROWTH PROMOTING PROTEIN FROM THE MOUSE SALIVARY GLAND AND ITS NEURO-CYTOTOXIC ANTISERUM.
    Proc Natl Acad Sci U S A. 1960 Mar;46(3):302-11 PMID: 16578483
  12. Neurotrophin-3: a neurotrophic factor related to NGF and BDNF.
    Science. 1990 Mar 23;247(4949 Pt 1):1446-51 PMID: 2321006
  13. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
    Science. 1985 Dec 20;230(4732):1350-4 PMID: 2999980
  14. Developmental changes of nerve growth factor and its mRNA in the rat hippocampus: comparison with choline acetyltransferase.
    Dev Biol. 1987 Apr;120(2):322-8 PMID: 2435590
  15. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  16. The emerging generality of the neurotrophic hypothesis.
    Trends Neurosci. 1988 Jun;11(6):243-4 PMID: 2465616
  17. Outgrowth of sympathetic adrenergic neurons in mice treated with a nerve-growth factor (NGF).
    Z Zellforsch Mikrosk Anat. 1967;81(2):155-73 PMID: 5628756
  18. Nerve growth factor in sympathetic ganglia and corresponding target organs of the rat: correlation with density of sympathetic innervation.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3513-6 PMID: 6407016
  19. Timing and site of nerve growth factor synthesis in developing skin in relation to innervation and expression of the receptor.
    Nature. 1987 Mar 26-Apr 1;326(6111):353-8 PMID: 3031505
  20. Trophic factors and neuronal survival.
    Neuron. 1989 Jun;2(6):1525-34 PMID: 2697237
  21. Relationship between levels of nerve growth factor (NGF) and its messenger RNA in sympathetic ganglia and peripheral target tissues.
    EMBO J. 1984 Dec 20;3(13):3183-9 PMID: 6549295
  22. Changes of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transection.
    J Cell Biol. 1987 Jun;104(6):1623-31 PMID: 3034917
  23. Expression of the beta-nerve growth factor gene correlates with the density of sympathetic innervation in effector organs.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7951-5 PMID: 6595669
  24. Nerve growth factors (NGF, BDNF) enhance axonal regeneration but are not required for survival of adult sensory neurons.
    J Neurosci. 1988 Jul;8(7):2394-405 PMID: 3249232
  25. Comparison between the time course of changes in nerve growth factor protein levels and those of its messenger RNA in the cultured rat iris.
    J Biol Chem. 1986 Jul 15;261(20):9246-9 PMID: 2424906
  26. In situ hybridization for the study of gene expression in the brain.
    Methods Enzymol. 1986;124:497-510 PMID: 3754925
  27. High-efficiency cloning of full-length cDNA; construction and screening of cDNA expression libraries for mammalian cells.
    Methods Enzymol. 1987;154:3-28 PMID: 2828856
  28. Survival and Axonal Elongation of Adult Rat Retinal Ganglion Cells.
    Eur J Neurosci. 1989 Jan;1(1):19-26 PMID: 12106170
  29. Nerve growth factor promotes survival of septal cholinergic neurons after fimbrial transections.
    J Neurosci. 1986 Aug;6(8):2155-62 PMID: 3746405
  30. Levels of nerve growth factor and its mRNA in the central nervous system of the rat correlate with cholinergic innervation.
    EMBO J. 1985 Jun;4(6):1389-93 PMID: 2411537
  31. Nerve growth factor gene expression in the developing rat brain.
    Science. 1986 Oct 17;234(4774):352-5 PMID: 3764415
  32. The physiological function of nerve growth factor in the central nervous system: comparison with the periphery.
    Rev Physiol Biochem Pharmacol. 1987;109:145-78 PMID: 3317757
  33. Nerve growth factor.
    Physiol Rev. 1968 Jul;48(3):534-69 PMID: 4875350
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-08-00
Pages
2459-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552273
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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