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PMID: 7890832 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

TrkA-immunoreactive profiles in the central nervous system: colocalization with neurons containing p75 nerve growth factor receptor, choline acetyltransferase, and serotonin.

The Journal of comparative neurology ·Vol. 350 ·No. 4 ·1994-12-22 ·Pages 587-611

Sobreviela T, Clary DO, Reichardt LF, Brandabur MM, Kordower JH, Mufson EJ

Abstract

The present investigation used an antibody directed against the extracellular domain of the signal transducing nerve growth factor receptor, trkA, to reveal immunoreactive perikarya or fibers within the olfactory bulb and tubercle, cingulate cortex, nucleus accumbens, striatum, endopiriform nucleus, septal/diagonal band complex, nucleus basalis, hippocampal complex, thalamic paraventricular and reuniens nuclei, periventricular hypothalamus, interpeduncular nucleus, mesencephalic nucleus of the fifth nerve, dorsal nucleus of the lateral lemniscus, prepositus hypoglossal nucleus, ventral cochlear nucleus, ventral lateral tegmentum, medial vestibular nucleus, spinal trigeminal nucleus oralis, nucleus of the solitary tract, raphe nuclei, and spinal cord. Colocalization experiments revealed that virtually all striatal trkA-immunoreactive neurons (> 99%) coexpressed choline acetyltransferase (ChAT) but not p75 nerve growth factor receptor (NGFR). Within the septal/diagonal band complex virtually all trkA neurons (> 95%) coexpressed both ChAT and p75 NGFR. More caudally, dual stained sections revealed numerous trkA/ChAT (> 80%) and trkA/p75 NGFR (> 95%) immunoreactive neurons within the nucleus basalis. In the brainstem, raphe serotonergic neurons (45%) coexpressed trkA. Sections stained with a pan-trk antibody that recognizes primarily trkA, as well as trkB and trkC, labeled neurons within all of these regions as well as within the hypothalamic arcuate, supramammilary, and supraoptic nuclei, hippocampus, inferior and superior colliculus, substantia nigra, ventral tegmental area of T'sai, and cerebellular Purkinje cells. Virtually all of these other regions with the exception of the cerebellum also expressed pan-trk immunoreactivity in the monkey. The widespread expression of trkA throughout the central neural axis suggests that this receptor may play a role in signal transduction mechanisms linked to NGF-related substances in cholinergic basal forebrain and noncholinergic systems. These findings suggest that pharmacological use of ligands for trkA could have beneficial effects on the multiple neuronal systems that are affected in such disorders as Alzheimer's disease.

Related Genes
trkA
MeSH Terms
Animals Central Nervous System/cytology,enzymology Choline O-Acetyltransferase/metabolism Immunohistochemistry Male Nerve Fibers/enzymology Neurons/enzymology,metabolism Prosencephalon/enzymology,physiology Protein-Tyrosine Kinases/metabolism Raphe Nuclei/enzymology,physiology Rats Rats, Sprague-Dawley Receptors, Nerve Growth Factor/metabolism Serotonin/metabolism Signal Transduction/physiology
Chemicals
Receptors, Nerve Growth Factor Serotonin Choline O-Acetyltransferase Protein-Tyrosine Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sobreviela T
Department of Neurological Sciences, Rush Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
Clary D O
Reichardt L F
Brandabur M M
Kordower J H
Mufson E J
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Article Info
Journal
The Journal of comparative neurology
Abbr.
J Comp Neurol
ISSN
0021-9967
DOI
10.1002/cne.903500407
Published
1994-12-22
Pages
587-611
Language
English
Region
United States
NLM ID
0406041
PMCID
PMC2710128
Subset
IM
Grants
NIA NIH HHS · R01 AG010668 · United States
NIA NIH HHS · AG10668 · United States
NIA NIH HHS · P01 AG009466 · United States
NIA NIH HHS · P30 AG010161 · United States
NINDS NIH HHS · P01 NS016033-17A10014 · United States
NIA NIH HHS · AG10161 · United States
NINDS NIH HHS · P01 NS016033 · United States
NIA NIH HHS · AG11482 · United States
External Links
PubMed source DOI: 10.1002/cne.903500407 PMC Full Text View journal details
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