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PMID: 8654371 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Differential V region mutation of two transfected Ig genes and their interaction in cultured B cell lines.

The EMBO journal ·Vol. 15 ·No. 11 ·1996-06-03 ·Pages 2738-47

Zhu M, Green NS, Rabinowitz JL, Scharff MD

Abstract

We have established B cell culture systems in which transfected and stably integrated Ig constructs spontaneously undergo high rates of variable (V) region mutation. Mutation rates were determined using reversion analysis of an Ig V region nonsense codon (Vn). A construct (Vn/gamma2a) in which a Vn was associated with the gamma2a constant region and its intervening and immediate flanking sequences mutated at a high rate of 2.2 x 10(-4)/bp/generation in the NSO myeloma cell line. This same Vn, when associated with the mu constant region (Vn/mu), mutated at a 1000-fold lower rate in NSO. The Vn/gamma2a construct also mutated at high rates in the 18.81 pre-B and the S107 myeloma cell lines and at a low rate in the J558 myeloma cell line. In NSO, the presence of the gamma2a construct raised the mutation rate of the mu construct and the mu decreased the mutation rate of gamma2a. These results suggest that there is both positive and negative regulation of V region mutation and that different cell lines express different combinations and/or amounts of trans-acting factors that are involved in the mutational process.

MeSH Terms
Animals B-Lymphocytes/immunology Cell Line Genes, Immunoglobulin Immunoglobulin G/genetics Immunoglobulin Variable Region/genetics Mice Point Mutation Transfection Tumor Cells, Cultured
Chemicals
Immunoglobulin G Immunoglobulin Variable Region
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zhu M
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Green N S
Rabinowitz J L
Scharff M D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-06-03
Pages
2738-47
Language
English
Region
England
NLM ID
8208664
PMCID
PMC450209
Subset
IM
Grants
NCI NIH HHS · CA39838 · United States
NIAID NIH HHS · P01AI33184 · United States
NCI NIH HHS · P01CA1330 · United States
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