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PMID: 2115570 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Heavy-chain class switch does not terminate somatic mutation.

The Journal of experimental medicine ·Vol. 172 ·No. 2 ·1990-08-01 ·Pages 531-6

Shan H, Shlomchik M, Weigert M

Abstract

We studied the H-chain class switch rearrangement in four groups of clonally related B cell hybridomas, to test the hypothesis that class switch terminates somatic mutation in a B cell. Using switch region-specific probes in Southern blot analysis individual mu-gamma switch rearrangement events can be distinguished. We show that clonally related IgG-producing hybridomas that differ by mutations often share a common switch rearrangement. This indicates that class switch in these cells did not terminate somatic mutation.

MeSH Terms
Animals Antibodies, Monoclonal Autoimmune Diseases/immunology Blotting, Southern Cell Line DNA/genetics,isolation & purification Gene Rearrangement Genes, Regulator Genes, Switch Immunoglobulin Heavy Chains/genetics Immunoglobulin Switch Region/genetics Liver/immunology Mice Mice, Mutant Strains Mutation Restriction Mapping
Chemicals
Antibodies, Monoclonal Immunoglobulin Heavy Chains DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shan H
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Shlomchik M
Weigert M
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34 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.172.2.531
Published
1990-08-01
Pages
531-6
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188341
Subset
IM
Grants
NCI NIH HHS · CA-06927 · United States
NIGMS NIH HHS · GM-20964 · United States
External Links
PubMed source DOI: 10.1084/jem.172.2.531 PMC Full Text View journal details
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