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PMID: 8603912 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Thioredoxin is required for vacuole inheritance in Saccharomyces cerevisiae.

The Journal of cell biology ·Vol. 132 ·No. 5 ·1996-03-00 ·Pages 787-94

Xu Z, Wickner W

Abstract

The vacuole of Saccharomyces cerevisiae projects a stream of tubules a and vesicles (a "segregation structure") into the bud in early S phase. We have described an in vitro reaction, requiring physiological temperature, ATP, and cytosol, in which isolated vacuoles form segregation structures and fuse. This in vitro reaction is defective when reaction components are prepared from vac mutants that are defective in this process in vivo, Fractionation of the cytosol reveals at least three components, each of which can support the vacuole fusion reaction, and two stimulatory fractions. Purification of one "low molecular weight activity" (LMA1) yields a heterodimeric protein with a thioredoxin subunit. Most of the thioredoxin of yeast is in this complex rather than the well-studied monomer. A deletion of both S. cerevisiae thioredoxin genes causes a striking vacuole inheritance defect in vivo, establishing a role for thioredoxin as a novel factor in this trafficking reaction.

MeSH Terms
Cell-Free System Cytosol/chemistry,metabolism Fungal Proteins/chemistry,genetics,metabolism Gene Deletion Membrane Fusion Protein Conformation Recombinant Proteins/metabolism Saccharomyces cerevisiae/genetics,growth & development,metabolism Thioredoxins/chemistry,genetics,metabolism Vacuoles/genetics,metabolism
Chemicals
Fungal Proteins Recombinant Proteins Thioredoxins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Xu Z
Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire, 03755-3844, USA.
Wickner W
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.132.5.787
Published
1996-03-00
Pages
787-94
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120747
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.132.5.787 PMC Full Text View journal details
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