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PMID: 1378448 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Nuclear and mitochondrial inheritance in yeast depends on novel cytoplasmic structures defined by the MDM1 protein.

The Journal of cell biology ·Vol. 118 ·No. 2 ·1992-07-00 ·Pages 385-95

McConnell SJ, Yaffe MP

Abstract

The mdml mutation causes temperature-sensitive growth and defective transfer of nuclei and mitochondria into developing buds of yeast cells at the nonpermissive temperature. The MDM1 gene was cloned by complementation, and its sequence revealed an open reading frame encoding a potential protein product of 51.5 kD. This protein displays amino acid sequence similarities to hamster vimentin and mouse epidermal keratin. Gene disruption demonstrated that MDM1 is essential for mitotic growth. Antibodies against the MDM1 protein recognized a 51-kD polypeptide that was localized by indirect immunofluorescence to a novel pattern of spots and punctate arrays distributed throughout the yeast cell cytoplasm. These structures disappeared after shifting mdm1 mutant cells to the nonpermissive temperature, although the cellular level of MDM1 protein was unchanged. Affinity-purified antibodies against MDM1 also specifically recognized intermediate filaments by indirect immunofluorescence of animal cells. These results suggest that novel cytoplasmic structures containing the MDM1 protein mediate organelle inheritance in yeast.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Cycle Proteins Cell Nucleus/physiology DNA, Fungal/genetics,isolation & purification Fluorescent Antibody Technique Fungal Proteins/analysis,genetics Genes, Fungal Genetic Complementation Test Intermediate Filament Proteins Keratins/genetics Mitochondria/physiology Molecular Sequence Data Molecular Weight Nocodazole/pharmacology Open Reading Frames Plasmids Restriction Mapping Saccharomyces cerevisiae/drug effects,genetics,ultrastructure Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid Vimentin/genetics
Chemicals
Cell Cycle Proteins DNA, Fungal Fungal Proteins Intermediate Filament Proteins MDM1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Vimentin Keratins Nocodazole
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McConnell S J
University of California, San Diego, Department of Biology, La Jolla 92093.
Yaffe M P
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.118.2.385
Published
1992-07-00
Pages
385-95
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2290041
Subset
IM
Databases
GENBANK
X66371
External Links
PubMed source DOI: 10.1083/jcb.118.2.385 PMC Full Text View journal details
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