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PMID: 2050111 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

vac2: a yeast mutant which distinguishes vacuole segregation from Golgi-to-vacuole protein targeting.

The EMBO journal ·Vol. 10 ·No. 7 ·1991-07-00 ·Pages 1741-8

Shaw JM, Wickner WT

Abstract

We have isolated four yeast mutants that are unable to partition maternal vacuoles into growing buds. Three of these vacuole segregation (vac) mutants also mislocalize the vacuolar protease carboxypeptidase Y (CPY) to the cell surface, a phenotype previously reported for vac strains. A fourth mutant, vac2-1, exhibits a temperature-sensitive defect in vacuole segregation but does not show a defect in protein targeting from the Golgi apparatus to the vacuole. Haploid vac2-1 cells grown at the non-permissive temperature do not secrete CPY or a second vacuolar protease, proteinase A (PrA). Furthermore, newly synthesized precursors of CPY are converted to mature forms with similar kinetics in both vac2-1 and wild-type cells. In addition, invertase is secreted normally from vac2-1 cells, indicating that post-Golgi steps in the secretory pathway are not blocked in this mutant. These results suggest that VAC2 function is necessary for vacuole division and segregation in yeast but is not involved in vacuole protein sorting events at the Golgi apparatus.

MeSH Terms
Aspartic Acid Endopeptidases/genetics,metabolism Biological Transport Carboxypeptidases/genetics,metabolism Cathepsin A Cell Nucleus/ultrastructure Fungal Proteins/genetics Golgi Apparatus/enzymology,microbiology,ultrastructure Kinetics Mitochondria/ultrastructure Mutation Saccharomyces cerevisiae/enzymology,genetics,growth & development Saccharomyces cerevisiae Proteins Temperature Vacuoles/enzymology,microbiology,ultrastructure
Chemicals
Fungal Proteins Saccharomyces cerevisiae Proteins Carboxypeptidases Cathepsin A PRC1 protein, S cerevisiae serine carboxypeptidase aspartic proteinase A Aspartic Acid Endopeptidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shaw J M
Molecular Biology Institute, University of California, Los Angeles 90024-1570.
Wickner W T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-07-00
Pages
1741-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452845
Subset
IM
Grants
NIGMS NIH HHS · GM13061 · United States
External Links
PubMed source PMC Full Text View journal details
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