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PMID: 8491780 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

HNF4 and HNF1 as well as a panel of hepatic functions are extinguished and reexpressed in parallel in chromosomally reduced rat hepatoma-human fibroblast hybrids.

The Journal of cell biology ·Vol. 121 ·No. 4 ·1993-05-00 ·Pages 887-98

Griffo G, Hamon-Benais C, Angrand PO, Fox M, West L, Lecoq O, Povey S, Cassio D, Weiss M

Abstract

Rat hepatoma-human fibroblast hybrids of two independent lineages containing only 8-11 human chromosomes show pleiotropic extinction of thirteen out of fifteen hepatic functions examined. Reexpression of the entire group of functions most often occurs in a block, and except for one discordant subclone, correlates with loss of human chromosome 2. The extinguished cells and their reexpressing derivatives have been examined for the expression of seven liver-enriched transcription factors. C/EBP, LAP, DBP, HNF3, and vHNF1 expression are not systematically extinguished in parallel with the hepatic functions. However, HNF1 and HNF4 show a perfect correlation with phenotype: these factors are expressed only in the cells showing pleiotropic reexpression. Since recent evidence indicates that HNF4 controls HNF1 expression, it can be proposed that the HNF4 gene is the primary target of the pleiotropic extinguisher.

MeSH Terms
Albumins/genetics Animals Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Blotting, Northern Cell Line Chromosomes/physiology Clone Cells DNA-Binding Proteins/genetics,metabolism Fibroblasts/cytology Gene Expression Regulation Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-alpha Hepatocyte Nuclear Factor 1-beta Hepatocyte Nuclear Factor 4 Humans Hybrid Cells Leucine Zippers Liver/metabolism Liver Neoplasms, Experimental Nuclear Proteins Phosphoproteins Rats Transcription Factors/genetics,metabolism Tumor Cells, Cultured
Chemicals
Albumins Basic Helix-Loop-Helix Leucine Zipper Transcription Factors DNA-Binding Proteins HNF1A protein, human HNF1B protein, human Hepatocyte Nuclear Factor 1-alpha Hepatocyte Nuclear Factor 4 Hnf1a protein, rat MLX protein, human Nuclear Proteins Phosphoproteins Transcription Factors Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-beta
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Griffo G
Unité de Génétique de la Différenciation, URA Centre National de la Recherche Scientifique 1149, Institut Pasteur, Paris, France.
Hamon-Benais C
Angrand P O
Fox M
West L
Lecoq O
Povey S
Cassio D
Weiss M
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.121.4.887
Published
1993-05-00
Pages
887-98
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119800
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.121.4.887 PMC Full Text View journal details
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