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PMID: 4308097 Published · ppublish English Journal Article

A quantitative comparison of formation of spontaneous and virus-produced viable hybrids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 62 ·No. 3 ·1969-03-00 ·Pages 852-9

Coon HG, Weiss MC

Abstract

Ultraviolet-inactivated Sendai virus used in conjunction with a selective system reproducibly gives high yields of viable hybrid cell lines. With two different crosses, the frequency of hybrid colonies obtained after virus treatment was found to be 100 times greater than the frequency of spontaneous hybrids, and this increase was found to show little variation between 33 and 1000 hemagglutinating units of virus. No differences have been found between the properties of hybrids obtained after Sendai treatment and those obtained from untreated mixed cultures of parental cells.

MeSH Terms
Animals Cell Line Clone Cells Connective Tissue Culture Media Culture Techniques Hybridization, Genetic L Cells Liver Mice Parainfluenza Virus 1, Human/radiation effects Radiation Genetics Rats Ultraviolet Rays
Chemicals
Culture Media
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Coon H G
Weiss M C
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.62.3.852
Published
1969-03-00
Pages
852-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC223676
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.62.3.852 PMC Full Text View journal details
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