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PMID: 8481004 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ScrFI restriction-modification system of Lactococcus lactis subsp. cremoris UC503: cloning and characterization of two ScrFI methylase genes.

Applied and environmental microbiology ·Vol. 59 ·No. 3 ·1993-03-00 ·Pages 777-85

Davis R, van der Lelie D, Mercenier A, Daly C, Fitzgerald GF

Abstract

Two genes from the total genomic DNA of dairy starter culture Lactococcus lactis subsp. cremoris UC503, encoding ScrFI modification enzymes, have been cloned and expressed in Escherichia coli. No homology between the two methylase genes was detected, and inverse polymerase chain reaction of flanking chromosomal DNA indicated that both were linked on the Lactococcus genome. Neither clone encoded the cognate endonuclease. The DNA sequence of one of the methylase genes (encoded by pCI931M) was determined and consisted of an open reading frame 1,170 bp long, which could encode a protein of 389 amino acids (M(r), 44.5). The amino acid sequence contained the highly characteristic motifs of an m5C methylase. Extensive regions of homology were observed with the methylases of NlaX, EcoRII, and Dcm.

Related Genes
ScrFI
MeSH Terms
Amino Acid Sequence Bacteriophages/genetics Base Sequence Blotting, Southern Cloning, Molecular DNA Modification Methylases/chemistry,genetics DNA, Bacterial/chemistry DNA-Cytosine Methylases/chemistry,genetics Deoxyribonucleases, Type II Site-Specific/chemistry,genetics Genes, Bacterial Lactococcus lactis/enzymology,genetics Molecular Sequence Data Nucleic Acid Hybridization Plasmids Polymerase Chain Reaction Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Alignment Sequence Homology, Amino Acid
Chemicals
DNA, Bacterial DNA Modification Methylases DNA-Cytosine Methylases ScrFI modification methylase CCNGG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Davis R
Department of Food Microbiology, University College, Cork, Ireland.
van der Lelie D
Mercenier A
Daly C
Fitzgerald G F
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.59.3.777-785.1993
Published
1993-03-00
Pages
777-85
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC202189
Subset
IM
Databases
GENBANK
L16019, L20867, M87289, X62981, X62986, X62987, X62988, X62989, X62990, X74289
External Links
PubMed source DOI: 10.1128/aem.59.3.777-785.1993 PMC Full Text View journal details
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