Home LiteratureArticle Details
PMID: 6298711 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ScrFI: a new sequence-specific endonuclease from Streptococcus cremoris.

Nucleic acids research ·Vol. 10 ·No. 24 ·1982-12-20 ·Pages 8171-9

Fitzgerlad GF, Daly C, Brown LR, Gingeras TR

Abstract

A novel sequence-specific endonuclease has been isolated from Streptococcus cremoris F. ScrFI recognises the sequence: (formula; see text) and cleaves as indicated by the arrow ( ). It is the first enzyme to recognise this sequence and the first endonuclease reported from the lactic streptococci used in dairy fermentations.

MeSH Terms
Base Sequence DNA Restriction Enzymes/isolation & purification,metabolism Deoxyribonucleases, Type II Site-Specific Electrophoresis, Agar Gel Streptococcus/enzymology Substrate Specificity
Chemicals
DNA Restriction Enzymes CCNGG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fitzgerlad G F
Daly C
Brown L R
Gingeras T R
References (15)
15 references, click to expand
  1. A new sequence-specific endonuclease from Streptococcus faecalis subsp. zymogenes.
    Gene. 1978 Dec;4(4):329-36 PMID: 105969
  2. A computer assisted method for the determination of restriction enzyme recognifion sites.
    Nucleic Acids Res. 1978 Nov;5(11):4105-27 PMID: 724510
  3. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. V. ON THE SPECIFICITY OF EXONUCLEASE I (PHOSPHODIESTERASE).
    J Biol Chem. 1964 Aug;239:2628-36 PMID: 14235546
  4. Restriction and modification in group N streptococci: effect of heat on development of modified lytic bacteriophage.
    Appl Environ Microbiol. 1980 Sep;40(3):500-6 PMID: 16345629
  5. Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA.
    Proc Natl Acad Sci U S A. 1973 Apr;70(4):1209-13 PMID: 4577794
  6. Multiple modification/restriction systems in lactic streptococci and their significance in defining a phage-typing system.
    J Dairy Res. 1980 Oct;47(3):401-9 PMID: 6778900
  7. Improved medium for lactic streptococci and their bacteriophages.
    Appl Microbiol. 1975 Jun;29(6):807-13 PMID: 16350018
  8. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.
    Biochem J. 1964 Jan;90(1):147-9 PMID: 5832284
  9. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.
    Eur J Biochem. 1969 Dec;11(2):395-9 PMID: 4902612
  10. Restriction endonucleases.
    CRC Crit Rev Biochem. 1976 Nov;4(2):123-64 PMID: 795607
  11. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.
    Proc Natl Acad Sci U S A. 1965 Jul;54(1):158-65 PMID: 5323016
  12. Purification and properties of the Bg1I and II endonucleases.
    Methods Enzymol. 1980;65(1):132-8 PMID: 6246333
  13. Purification and properties of the BamHI endonuclease.
    Methods Enzymol. 1980;65(1):147-53 PMID: 6246335
  14. Two sequence-specific endonucleases from Moraxella bovis.
    J Mol Biol. 1977 Jul;114(1):169-79 PMID: 909085
  15. DNA modification and restriction.
    Prog Nucleic Acid Res Mol Biol. 1974;14(0):1-37 PMID: 4602489
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.24.8171
Published
1982-12-20
Pages
8171-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327077
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.24.8171 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2