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PMID: 8468344 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A cytoplasmically anchored nuclear protein interferes specifically with the import of nuclear proteins but not U1 snRNA.

The Journal of cell biology ·Vol. 121 ·No. 2 ·1993-04-00 ·Pages 229-40

van Zee K, Dickmanns A, Fischer U, Lührmann R, Fanning E

Abstract

A cytoplasmically anchored mutant SV40 T antigen, FS T antigen, was shown previously to interfere specifically with the nuclear import of a heterologous nuclear protein, adenovirus 5 fiber protein, in cultured monkey cells (Schneider, J., C. Schindewolf, K. van Zee, and E. Fanning. 1988. Cell. 54:117-125; van Zee, K., F. Appel, and E. Fanning. 1991. Mol. Cell. Biol. 11:5137-5146). In this report, we demonstrate that FS T antigen also interferes with the nuclear import of adenovirus E1A and a peptide-albumin conjugate bearing multiple copies of the T antigen nuclear localization signal, but not with the import of U1 snRNA. A kinetic analysis indicates that nuclear import of the albumin-peptide conjugate is inhibited only when high intracellular concentrations of FS T antigen are reached. After microinjection into the cytoplasm of cultured cells, purified FS T antigen protein does not accumulate at the nuclear periphery, but rather is distributed in a punctate pattern throughout the cytoplasm. These data support a model in which cytoplasmic anchoring of FS T antigen enables the mutant protein to sequester and titrate out a cellular factor which is required for nuclear protein but not U1 snRNA import.

MeSH Terms
Adenovirus E1A Proteins/metabolism Amino Acid Sequence Animals Antigens, Viral, Tumor/analysis,genetics,pharmacology Cell Line/metabolism Cytoplasm/chemistry,drug effects,metabolism HeLa Cells Humans Kinetics Molecular Sequence Data Mutation Nuclear Proteins/metabolism Ribonucleoprotein, U1 Small Nuclear/metabolism Vero Cells
Chemicals
Adenovirus E1A Proteins Antigens, Viral, Tumor Nuclear Proteins Ribonucleoprotein, U1 Small Nuclear
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
van Zee K
Institut für Biochemie, Munich, Federal Republic of Germany.
Dickmanns A
Fischer U
Lührmann R
Fanning E
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.121.2.229
Published
1993-04-00
Pages
229-40
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2200110
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.121.2.229 PMC Full Text View journal details
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