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PMID: 1656223 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A hydrophobic protein sequence can override a nuclear localization signal independently of protein context.

Molecular and cellular biology ·Vol. 11 ·No. 10 ·1991-10-00 ·Pages 5137-46

van Zee K, Appel F, Fanning E

Abstract

Simian virus 40 T antigen is specifically targeted to the nucleus by the signal Pro-Lys-Lys-128-Lys-Arg-Lys-Val. We have previously described the isolation of a simian virus 40 T-antigen mutant, 676FS, which retains a wild-type nuclear localization signal but fails to accumulate properly in the nucleus and interferes with the nuclear localization of heterologous proteins. Here we report that the hydrophobic carboxy-terminal sequence novel to 676FS T antigen overrides the nuclear localization signal if fused to other proteins, thereby anchoring the proteins in the cytoplasm. We discuss possible mechanisms by which missorting of such a fusion protein could interfere with the nuclear transport of heterologous proteins.

MeSH Terms
Amino Acid Sequence Antigens, Polyomavirus Transforming/chemistry,genetics,metabolism Cell Line Cell Nucleus/metabolism Fluorescent Antibody Technique Molecular Sequence Data Mutation/genetics Nuclear Proteins/metabolism Protein Sorting Signals/metabolism Recombinant Fusion Proteins/metabolism Simian virus 40/genetics,metabolism beta-Galactosidase/genetics
Chemicals
Antigens, Polyomavirus Transforming Nuclear Proteins Protein Sorting Signals Recombinant Fusion Proteins beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
van Zee K
Institute for Biochemistry, Munich, Germany.
Appel F
Fanning E
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56 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.10.5137-5146.1991
Published
1991-10-00
Pages
5137-46
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361531
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.11.10.5137-5146.1991 PMC Full Text View journal details
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