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PMID: 8428766 Published · ppublish English Journal Article

The mouse Eb meiotic recombination hotspot contains a tissue-specific transcriptional enhancer.

Immunogenetics ·Vol. 37 ·No. 5 ·1993-00-00 ·Pages 331-6

Ling X, Shenkar R, Sakai D, Arnheim N

Abstract

A meiotic recombination hotspot exists within the second intron of the mouse major histocompatibility complex (MHC) gene, Eb. In the present study, a small fragment from the intron which contains two potential transcriptional regulatory elements was cloned into an expression vector and its effect on transcription was tested. This fragment was found to contain tissue-specific transcriptional enhancer activity. An octamer-like sequence and a B motif may contribute to this enhancer activity. Similar regulatory sequences with the same orientation and distance from one another are found in another mouse MHC recombination hotspot.

Related Genes
Eb
MeSH Terms
Animals Base Sequence Cell Line Enhancer Elements, Genetic/genetics Fibroblasts Genes, MHC Class II/genetics Meiosis/genetics Mice Mice, Inbred C3H Molecular Sequence Data Plasmids/genetics RNA, Messenger/genetics Recombination, Genetic/genetics Transfection Tumor Cells, Cultured
Chemicals
RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ling X
Molecular Biology Section, University of Southern California, Los Angeles 90089-1340.
Shenkar R
Sakai D
Arnheim N
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00216797
Published
1993-00-00
Pages
331-6
Language
English
Region
United States
NLM ID
0420404
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00216797 View journal details
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