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PMID: 8376330 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Regulation of the pcaIJ genes for aromatic acid degradation in Pseudomonas putida.

Journal of bacteriology ·Vol. 175 ·No. 18 ·1993-09-00 ·Pages 5829-38

Parales RE, Harwood CS

Abstract

Six of the genes encoding enzymes of the beta-ketoadipate pathway for benzoate and 4-hydroxybenzoate degradation in Pseudomonas putida are organized into at least three separate transcriptional units. As an initial step to defining this pca regulon at the molecular level, lacZ fusions were made with the pcaI and pcaJ genes, which encode the two subunits of beta-ketoadipate:succinyl-coenzyme A transferase, the enzyme catalyzing the next-to-last step in the beta-ketoadipate pathway. Fusion analyses showed that pcaI and pcaJ constitute an operon which requires beta-ketoadipate or its nonmetabolizable analog, adipate, as well as the pcaR regulatory gene for induction. The pcaIJ promoter is likely to be a sigma 70-type promoter; it has a sigma 70-type consensus sequence and did not require the alternative sigma factor, RpoN, for induction. Deletion analysis of the promoter region of a pcaI-lacZ transcriptional fusion indicated that no specific DNA sequences upstream of the -35 region were required for full induction. This implies that the binding site for the activator protein, PcaR, is unusually close to the transcriptional start site of pcaIJ.

Related Genes
PcaR pcaI pcaJ
MeSH Terms
Adipates/metabolism Base Sequence Cloning, Molecular Coenzyme A-Transferases/genetics DNA, Bacterial Escherichia coli/genetics Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Genes, Bacterial Genes, Regulator Glucose/metabolism Molecular Sequence Data Molecular Structure Operator Regions, Genetic Operon Promoter Regions, Genetic Pseudomonas putida/genetics Recombinant Fusion Proteins/genetics Restriction Mapping Succinates/metabolism Succinic Acid
Chemicals
Adipates DNA, Bacterial Recombinant Fusion Proteins Succinates 3-oxoadipic acid Succinic Acid Coenzyme A-Transferases 3-oxoadipate CoA-transferase Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Parales R E
Department of Microbiology, University of Iowa, Iowa City 52242.
Harwood C S
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39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.18.5829-5838.1993
Published
1993-09-00
Pages
5829-38
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206662
Subset
IM
Grants
NIGMS NIH HHS · GM08365 · United States
External Links
PubMed source DOI: 10.1128/jb.175.18.5829-5838.1993 PMC Full Text View journal details
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