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PMID: 8360178 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Purification of the major histocompatibility complex class I transcription factor H2TF1. The full-length product of the nfkb2 gene.

The Journal of biological chemistry ·Vol. 268 ·No. 25 ·1993-09-05 ·Pages 18882-90

Potter DA, Larson CJ, Eckes P, Schmid RM, Nabel GJ, Verdine GL, Sharp PA

Abstract

H2TF1 is a ubiquitous major histocompatibility complex (MHC) class I-specific transcription factor, which binds to the palindromic kappa B enhancer site upstream of MHC class I genes. Here we report that H2TF1 consists of a polypeptide with relative molecular mass 110,000, that corresponds to the predicted 100-kDa product (NF-kappa B2 p100) encoded by the candidate proto-oncogene nfkb2 (lyt-10). H2TF1 was purified by a novel affinity chromatography method and identified as the NF-kappa B2 p100 polypeptide by peptide sequencing as well as by reactivity with a specific antiserum. Purified H2TF1 binds the MHC kappa B site with high affinity (KD = 3 x 10(-11) M), in contrast with previous reports that NF-kappa B2 p100 did not bind DNA.

Related Genes
nfkb2
MeSH Terms
Amino Acid Sequence Base Sequence Binding Sites Chromatography, Affinity DNA/chemistry,metabolism Enhancer Elements, Genetic Genes, MHC Class I HeLa Cells/chemistry Humans Molecular Sequence Data Molecular Weight NF-kappa B NF-kappa B p52 Subunit Photochemistry Proto-Oncogene Mas Sequence Analysis Transcription Factors/chemistry,isolation & purification,metabolism Ultraviolet Rays
Chemicals
MAS1 protein, human NF-kappa B NF-kappa B p52 Subunit Proto-Oncogene Mas Transcription Factors DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Potter D A
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Larson C J
Eckes P
Schmid R M
Nabel G J
Verdine G L
Sharp P A
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
Published
1993-09-05
Pages
18882-90
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC2758046
Subset
IM
Grants
NCI NIH HHS · K08 CA001562-04 · United States
NCI NIH HHS · K08-CA-01562-01 · United States
NCI NIH HHS · P01 CA042063 · United States
NCI NIH HHS · P30 CA014051 · United States
NCI NIH HHS · P30-CA14051 · United States
NCI NIH HHS · P01-CA42063 · United States
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PubMed source PMC Full Text View journal details
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