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PMID: 1732739 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RelB, a new Rel family transcription activator that can interact with p50-NF-kappa B.

Molecular and cellular biology ·Vol. 12 ·No. 2 ·1992-02-00 ·Pages 674-84

Ryseck RP, Bull P, Takamiya M, Bours V, Siebenlist U, Dobrzanski P, Bravo R

Abstract

We have identified a serum-inducible gene, relB, which encodes a protein of 558 amino acids containing a region with high similarity to c-Rel and other members of the Rel family. Transcriptional activation analysis of GAL4-RelB fusion proteins in yeast cells reveals that RelB contains in its C-terminal 180 amino acids a transcriptional activation domain. The N-terminal part including the region of similarity with the Rel family shows no detectable transcriptional activity. RelB does not bind with high affinity to NF-kappa B sites, but heterodimers between RelB and p50-NF-kappa B do bind to different NF-kappa B-binding sites with a similar affinity to that shown by p50-NF-kappa B homodimers. However, RelB/p50-NF-kappa B heterodimers, in contrast to p50-NF-kappa B homodimers, transactivate transcription of a promoter containing a kappa B-binding site.

Related Genes
relB
MeSH Terms
3T3 Cells Amino Acid Sequence Animals Base Sequence DNA-Binding Proteins Fungal Proteins/genetics,metabolism Gene Expression Regulation/physiology Mice Molecular Sequence Data Multigene Family/genetics NF-kappa B/metabolism Oncogene Proteins v-rel Oncogenes/genetics Precipitin Tests Recombinant Fusion Proteins/genetics,metabolism Retroviridae Proteins, Oncogenic/chemistry,genetics,metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription Factors/metabolism beta-Galactosidase/genetics,metabolism
Chemicals
DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae NF-kappa B Oncogene Proteins v-rel Recombinant Fusion Proteins Retroviridae Proteins, Oncogenic Saccharomyces cerevisiae Proteins Transcription Factors beta-Galactosidase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ryseck R P
Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000.
Bull P
Takamiya M
Bours V
Siebenlist U
Dobrzanski P
Bravo R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.2.674-684.1992
Published
1992-02-00
Pages
674-84
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364256
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.12.2.674-684.1992 PMC Full Text View journal details
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