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PMID: 8327482 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An ordered collection of Bacillus subtilis DNA segments cloned in yeast artificial chromosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 13 ·1993-07-01 ·Pages 6047-51

Azevedo V, Alvarez E, Zumstein E, Damiani G, Sgaramella V, Ehrlich SD, Serror P

Abstract

A collection of 772 Bacillus subtilis DNA segments was obtained by cloning in yeast artificial chromosomes. The B. subtilis inserts of 288 clones were mapped by hybridization using as probes 65 cloned genes and 188 isolated insert ends. In this way, 59 inserts were ordered in four contigs that cover > 98% of the B. subtilis chromosome. This ordered collection is now available for further genetic and physical analysis of the B. subtilis genome.

MeSH Terms
Bacillus subtilis/genetics Base Sequence Chromosome Mapping Chromosomes, Bacterial Chromosomes, Fungal Cloning, Molecular DNA, Bacterial/genetics Genes, Bacterial Molecular Sequence Data
Chemicals
DNA, Bacterial
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Azevedo V
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.
Alvarez E
Zumstein E
Damiani G
Sgaramella V
Ehrlich S D
Serror P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.13.6047
Published
1993-07-01
Pages
6047-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46864
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.90.13.6047 PMC Full Text View journal details
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