Abstract
Eight mutants of Bacillus subtilis deficient in xylan utilization were isolated and characterized genetically and biochemically. Each mutant was obtained independently after nitrosoguanidine mutagenesis. All of the analyzed mutations were shown to be linked. Reciprocal transformation crosses revealed the existence of two genes controlling xylan utilization which have been designated xynA and xynB. Available data have indicated that these two genes code for two xylan-degrading enzymes existing in the wild-type strains, an extracellular beta-xylanase (xynA) and a cell-associated beta-xylosidase (xynB).
MeSH Terms
Bacillus subtilis/enzymology,genetics,metabolism
Chromosome Mapping
Chromosomes, Bacterial
Genes, Bacterial
Glycoside Hydrolases/genetics
Mutation
Polysaccharides/metabolism
Transduction, Genetic
Transformation, Bacterial
Xylan Endo-1,3-beta-Xylosidase
Xylans/metabolism
Xylosidases/genetics
Chemicals
Polysaccharides
Xylans
Glycoside Hydrolases
Xylosidases
Xylan Endo-1,3-beta-Xylosidase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Roncero M I
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