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PMID: 2477242 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of multiple HTF-island associated genes in the human major histocompatibility complex class III region.

The EMBO journal ·Vol. 8 ·No. 8 ·1989-08-00 ·Pages 2305-12

Sargent CA, Dunham I, Campbell RD

Abstract

Chromosome walking in the major histocompatibility complex (MHC) class III region has resulted in the isolation of 541 kb of genomic DNA in two sets of overlapping cosmid clones. These two sets encompass the 340 kb separating the C2 and tumour necrosis factor (TNF) alpha and beta genes, except for a 22 kb gap 108 kb centromeric to the TNF alpha gene. The genomic DNA inserts have been characterized for the presence of clusters of restriction sites with CpG dinucleotides in their recognition sequence. In conjunction with pulsed field gel electrophoresis the exact sites which cleave in chromosomal DNA have been established and this has suggested the presence of a number of HTF-islands. Genomic probes flanking the HTF-islands have been hybridized to Northern blots of RNA from a number of cell lines. Transcripts ranging in size from 0.6 to 6 kb corresponding to the products of 12 novel, single copy genes have been identified. In addition the human equivalent of the murine B144 gene was mapped approximately 10 kb centromeric of the TNF alpha gene. The location of so many new genes in this region raises the question as to whether they play any role in the observed HLA associations with an individual's susceptibility to develop autoimmune disease.

MeSH Terms
Base Sequence Blotting, Northern Blotting, Southern Cells, Cultured Complement C2/genetics Cosmids Cytosine Nucleotides/genetics DNA/genetics Deoxyribonuclease HpaII Deoxyribonucleases, Type II Site-Specific/metabolism Genetic Linkage Guanine Nucleotides/genetics Humans Major Histocompatibility Complex Multigene Family Oligonucleotides RNA, Messenger/genetics Restriction Mapping Transcription, Genetic Tumor Necrosis Factor-alpha/genetics
Chemicals
Complement C2 Cytosine Nucleotides Guanine Nucleotides Oligonucleotides RNA, Messenger Tumor Necrosis Factor-alpha DNA Deoxyribonuclease HpaII Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sargent C A
MRC Immunochemistry Unit, University of Oxford, UK.
Dunham I
Campbell R D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-08-00
Pages
2305-12
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401163
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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