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PMID: 3871526 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Mapping of steroid 21-hydroxylase genes adjacent to complement component C4 genes in HLA, the major histocompatibility complex in man.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 2 ·1985-01-00 ·Pages 521-5

Carroll MC, Campbell RD, Porter RR

Abstract

The genes for four components (C) of complement in the human major histocompatibility complex (HLA) have been aligned previously in a series of overlapping cosmid cloned inserts. Those inserts, which contained the two C4 genes C4A and C4B, hybridized with human adrenal mRNA, indicating that they contain a gene expressed in the adrenal. The mRNA fraction of 2.4 kilobases (kb) hybridizes with genomic DNA of 4.5 kb, which is duplicated and lies about 1.5 kb 3' of both the C4A and the C4B complement genes. Sequencing of a 430-base section and comparison with the published cDNA sequence of bovine cytochrome P-450 21-hydroxylase, peptide sequences of porcine 21-hydroxylase, and a cDNA sequence of a rat liver cytochrome P-450 identified the gene as coding for human steroid 21-hydroxylase [steroid,hydrogen-donor:oxygen oxidoreductase (21-hydroxylating), EC 1.14.99.10]. Mapping of the gene was helped by use of a synthetic oligonucleotide based on the bovine cDNA sequence.

MeSH Terms
Adrenal Glands/analysis Amino Acid Sequence Animals Base Sequence Cattle Cloning, Molecular Complement C4/genetics Complement C4a Complement C4b DNA/analysis Humans Major Histocompatibility Complex Nucleic Acid Hybridization RNA, Messenger/analysis Rats Steroid 21-Hydroxylase/genetics Steroid Hydroxylases/genetics Swine
Chemicals
Complement C4 RNA, Messenger Complement C4a Complement C4b DNA Steroid Hydroxylases Steroid 21-Hydroxylase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Carroll M C
Campbell R D
Porter R R
References (19)
19 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. Structure and organization of the C4 genes.
    Philos Trans R Soc Lond B Biol Sci. 1984 Sep 6;306(1129):379-88 PMID: 6149580
  3. HLA-linked congenital adrenal hyperplasia results from a defective gene encoding a cytochrome P-450 specific for steroid 21-hydroxylation.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7505-9 PMID: 6334310
  4. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  5. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  6. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  7. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  8. The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.
    Nucleic Acids Res. 1981 Feb 25;9(4):879-94 PMID: 7232206
  9. Polymorphic DNA region adjacent to the 5' end of the human insulin gene.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5759-63 PMID: 6272317
  10. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  11. Primary structure of a cytochrome P-450: coding nucleotide sequence of phenobarbital-inducible cytochrome P-450 cDNA from rat liver.
    Proc Natl Acad Sci U S A. 1982 May;79(9):2793-7 PMID: 6953431
  12. The construction of cosmid libraries which can be used to transform eukaryotic cells.
    Nucleic Acids Res. 1982 Nov 11;10(21):6715-32 PMID: 6294612
  13. Cloning of a human complement component C4 gene.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):264-7 PMID: 6572000
  14. Steroid 21-hydroxylase (cytochrome P-450) from porcine adrenocortical microsomes: microsequence analysis of cysteine-containing peptides.
    Biochemistry. 1983 Jan 4;22(1):143-9 PMID: 6830757
  15. Report of the Committee on the Genetic Constitution of Chromosome 6.
    Cytogenet Cell Genet. 1984;37(1-4):47-70 PMID: 6360564
  16. A molecular map of the human major histocompatibility complex class III region linking complement genes C4, C2 and factor B.
    Nature. 1984 Jan 19-25;307(5948):237-41 PMID: 6559257
  17. The structural basis of the multiple forms of human complement component C4.
    Cell. 1984 Apr;36(4):907-14 PMID: 6546707
  18. Cloning and expression of cDNA encoding a bovine adrenal cytochrome P-450 specific for steroid 21-hydroxylation.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1986-90 PMID: 6609358
  19. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.2.521
Published
1985-01-00
Pages
521-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397071
Subset
IM
Databases
GENBANK
K02771
External Links
PubMed source DOI: 10.1073/pnas.82.2.521 PMC Full Text View journal details
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