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PMID: 8114696 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Species-specific interaction of the glutamine-rich activation domains of Sp1 with the TATA box-binding protein.

Molecular and cellular biology ·Vol. 14 ·No. 3 ·1994-03-00 ·Pages 1582-93

Emili A, Greenblatt J, Ingles CJ

Abstract

We have used protein-blotting and protein affinity chromatography to demonstrate that each of the two glutamine-rich activation domains of the human transcription factor Sp1 can bind specifically and directly to the C-terminal evolutionarily conserved domain of the human TATA box-binding protein (TBP). These activation domains of Sp1 also bind directly to Drosophila TBP but bind much less strongly to TBP from the yeast Saccharomyces cerevisiae. The abilities of the Sp1 activation domains to interact directly with the TBPs of various species correlate well with their abilities to activate transcription in extracts derived from the same species. We also show that a glutamine-rich transcriptional activating region of the Drosophila protein Antennapedia binds directly to TBP in a species-specific manner that reflects its ability to activate transcription in vivo. These results support the notion that TBP is a direct and important target of glutamine-rich transcriptional activators.

Related Genes
Antp
MeSH Terms
Animals Antennapedia Homeodomain Protein Binding Sites DNA-Binding Proteins/metabolism Drosophila Proteins Drosophila melanogaster Fungal Proteins/metabolism Glutamine/chemistry HeLa Cells Homeodomain Proteins Humans Macromolecular Substances Nuclear Proteins Protein Binding Recombinant Fusion Proteins Saccharomyces cerevisiae Sp1 Transcription Factor/metabolism Species Specificity TATA-Box Binding Protein Transcription Factors/metabolism Transcriptional Activation
Chemicals
Antennapedia Homeodomain Protein Antp protein, Drosophila DNA-Binding Proteins Drosophila Proteins Fungal Proteins Homeodomain Proteins Macromolecular Substances Nuclear Proteins Recombinant Fusion Proteins Sp1 Transcription Factor TATA-Box Binding Protein Transcription Factors Glutamine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Emili A
Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.
Greenblatt J
Ingles C J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.3.1582-1593.1994
Published
1994-03-00
Pages
1582-93
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358517
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.3.1582-1593.1994 PMC Full Text View journal details
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