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PMID: 1502179 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Simple derivation of TFIID-dependent RNA polymerase II transcription systems from Schizosaccharomyces pombe and other organisms, and factors required for transcriptional activation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 16 ·1992-08-15 ·Pages 7659-63

Flanagan PM, Kelleher RJ, Tschochner H, Sayre MH, Kornberg RD

Abstract

Resolution of whole cell extract through two chromatographic steps yields a single protein fraction requiring only the addition of TFIID for the initiation of transcription at RNA polymerase II promoters. This approach allows the convenient generation of RNA polymerase II transcription systems from Saccharomyces cerevisiae, human lymphocytes, and Schizosaccharomyces pombe. TFIIDs from all three organisms are interchangeable among all three systems. The S. cerevisiae and Sch. pombe systems support effects of acidic activator proteins, provided a further protein fraction from S. cerevisiae is supplied. This further fraction is distinct from the mediator of transcriptional activation described previously and represents a second component in addition to general initiation factors that may facilitate a response to acidic activators.

MeSH Terms
Cell Line Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Humans Kinetics Lymphocytes Promoter Regions, Genetic RNA Polymerase II/genetics Saccharomyces cerevisiae/genetics,metabolism Schizosaccharomyces/genetics,metabolism Transcription Factor TFIID Transcription Factors/isolation & purification,metabolism Transcription, Genetic
Chemicals
Transcription Factor TFIID Transcription Factors RNA Polymerase II
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Flanagan P M
Department of Cell Biology, Fairchild Center, Stanford University School of Medicine, CA 94305.
Kelleher R J
Tschochner H
Sayre M H
Kornberg R D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.16.7659
Published
1992-08-15
Pages
7659-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49770
Subset
IM
Grants
NIGMS NIH HHS · GM36659 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.16.7659 PMC Full Text View journal details
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