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PMID: 809474 Published · ppublish English Journal Article

Carbohydrate fermentation plate medium for confirmation of Neisseria species.

Journal of clinical microbiology ·Vol. 1 ·No. 3 ·1975-03-00 ·Pages 294-7

Faur YC, Weisburd MH, Wilson ME

Abstract

A carbohydrate fermentation plate medium is described for rapid and reliable confirmation of Neisseria gonorrhoeae, N. meningitidis, and other Neisseria species. The medium is based on a modification of NYC (U.S. Patent 3,846,241) medium, originally designed for the isolation of pathogenic Neisseria (3). A total of 715 clinical isolates were tested for their carbohydrate fermentation reactions on the medium, in parallel with cystin-Trypticase agar medium. In 82% of the strains tested, results were in agreement on both media and 18% gave conflicting results. The NYC modification provides rapid and accurate fermentation patterns for use in routine confirmation procedures for Neisseria species.

MeSH Terms
Blood/microbiology Carbohydrate Metabolism Cerebrospinal Fluid/microbiology Culture Media Eye/microbiology Fermentation Humans Neisseria/isolation & purification,metabolism Neisseria gonorrhoeae/isolation & purification Neisseria meningitidis/isolation & purification Pharynx/microbiology Sputum/microbiology Synovial Fluid/microbiology Urogenital System/microbiology
Chemicals
Culture Media
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Faur Y C
Weisburd M H
Wilson M E
References (10)
10 references, click to expand
  1. LABORATORY DIAGNOSIS OF GONOCOCCAL INFECTIONS.
    Bull World Health Organ. 1965;32:449-69 PMID: 14315716
  2. A new fermentation medium for N. gonorrhoeae, HAP-medium. Influence of different constituents on growth and indicator colour.
    Acta Pathol Microbiol Scand. 1963;58:51-71 PMID: 13957984
  3. The rapid detection of lactose fermentation in paracolon organisms by the demonstration of beta-D-galactosidase.
    J Med Lab Technol. 1962 Jan;19:21-5 PMID: 14466934
  4. Carbohydrate fermentation patterns of Neisseria meningitidis determined by a microtiter method.
    Appl Microbiol. 1971 Jun;21(6):1072-4 PMID: 4327611
  5. An improved fermentation medium for Neisseria gonorrhoeae and other Neisseria.
    Health Lab Sci. 1965 Oct;2(4):238-41 PMID: 4953822
  6. A serum-free medium for testing fermentation reactions in Neisseria gonorrhoeae.
    J Clin Pathol. 1972 Jun;25(6):525-7 PMID: 4625435
  7. Gonorrhea--diagnosis and treatment.
    Obstet Gynecol. 1972 Feb;39(2):274-85 PMID: 4621637
  8. Rapid fermentation confirmation of Neisseria gonorrhoeae.
    Appl Microbiol. 1973 Apr;25(4):550-2 PMID: 4633478
  9. Modification of the rapid fermentation test for Neisseria gonorrhoeae.
    Appl Microbiol. 1974 Jun;27(6):1027-30 PMID: 4598431
  10. Simplified sugar fermentation plate technique for identification of Neisseria gonorrhoeae.
    Appl Microbiol. 1974 Feb;27(2):416-7 PMID: 4207283
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
Published
1975-03-00
Pages
294-7
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC275063
Subset
IM
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