Rapid fermentation confirmation of Neisseria gonorrhoeae.

Applied microbiology ·Vol. 25 ·No. 4 ·1973-04-00 ·Pages 550-2

Kellogg DS, Turner EM

Abstract

A rapid sugar fermentation procedure has been developed for the confirmation of Neisseria gonorrhoeae from either primary isolation media or purification media. A lightly buffered salt solution with pH indicator and sugars was heavily inoculated with presumptively positive growth. Proper fermentation patterns are obtained in 1 to 4 h with no interference from inhibited contaminants or variation in results due to differing growth requirements of gonococcal strains.

MeSH Terms

Bacteriological Techniques Culture Media Diagnosis, Differential Fermentation Fluorescent Antibody Technique Fructose/metabolism Glucose/metabolism Gonorrhea/diagnosis Humans Hydrogen-Ion Concentration Maltose/metabolism Methods Neisseria gonorrhoeae/classification,growth & development,isolation & purification,metabolism

Chemicals

Culture Media Fructose Maltose Glucose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kellogg D S

Turner E M

References (8)

8 references, click to expand

Asymptomatic gonorrhea.
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Fermentations: biochemical micromethods for bacteriology.
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Pseudomonas aeruginosa: Its Rôle as a Plant Pathogen.
J Bacteriol. 1942 Dec;44(6):633-45 PMID: 16560603
A SELECTIVE MEDIUM FOR THE CULTIVATION OF N. GONORRHOEAE AND N. MENINGITIDIS.
Public Health Rep. 1964 Jan;79:49-57 PMID: 14105729
Neisseria gonorrhoeae. II. Colonial variation and pathogenicity during 35 months in vitro.
J Bacteriol. 1968 Sep;96(3):596-605 PMID: 4979098
An improved fermentation medium for Neisseria gonorrhoeae and other Neisseria.
Health Lab Sci. 1965 Oct;2(4):238-41 PMID: 4953822
Identification of Neisseria meningitidis carbohydrate fermentation patterns in Mueller-Hinton broth.
J Bacteriol. 1968 Aug;96(2):563 PMID: 4970654
NEISSERIA GONORRHOEAE IDENTIFICATION IN DIRECT SMEARS BY A FLUORESCENT ANTIBODY-COUNTERSTAIN METHOD.
Appl Microbiol. 1965 Mar;13:171-4 PMID: 14325874

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.25.4.550-552.1973

Published

1973-04-00

Pages

550-2

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC380859

Subset

External Links

PubMed source DOI: 10.1128/am.25.4.550-552.1973 PMC Full Text

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