Carbohydrate fermentation patterns of Neisseria meningitidis determined by a microtiter method.

Applied microbiology ·Vol. 21 ·No. 6 ·1971-06-00 ·Pages 1072-4

Davies JA, Mitzel JR, Beam WE

Abstract

A carbohydrate fermentation technique has been developed and compared to the standard fermentation test with cystine-Trypticase-semisolid agar for the identification of Neisseria meningitidis. This new method utilizes Mueller-Hinton broth as a basal substrate and is performed with microtiter methods. By using Mueller-Hinton broth with and without the addition of antibiotics, the method can be adjusted to test the fermentation patterns of all of the Neisseria including N. gonorrhoeae.

MeSH Terms

Agar Agglutination Tests Bacteriological Techniques Carbohydrate Metabolism Colorimetry Culture Media Cystine Fermentation Hydrogen-Ion Concentration Methods Neisseria/isolation & purification Neisseria gonorrhoeae/isolation & purification Neisseria meningitidis/growth & development,isolation & purification,metabolism Phenolphthaleins Polymyxins Ristocetin Species Specificity Trypsin

Chemicals

Culture Media Phenolphthaleins Polymyxins Ristocetin Cystine Agar Trypsin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Davies J A

Mitzel J R

Beam W E

References (7)

7 references, click to expand

Variations in the fermentative capacity of neisseriae.
Am J Clin Pathol. 1950 Jul;20(7):630-6 PMID: 15432359
Proposed model for screening antimicrobial agents for potential use in eliminating meningococci from the nasopharynx of healthy carriers.
Antimicrob Agents Chemother (Bethesda). 1968;8:307-14 PMID: 4980835
LACTOSE-FERMENTING ORGANISMS RESEMBLING NEISSERIA MENINGITIDIS.
J Bacteriol. 1965 Aug;90:560 PMID: 14329479
An improved fermentation medium for Neisseria gonorrhoeae and other Neisseria.
Health Lab Sci. 1965 Oct;2(4):238-41 PMID: 4953822
Relationship of Serogroups of Neisseria meningitidis I. Microagglutination, Gel Diffusion, and Slide Agglutination Studies of Meningococcal Antisera Before and After Absorption with RAS-10 Strain of Meningococci.
Infect Immun. 1970 Mar;1(3):226-31 PMID: 16557721
Identification of Neisseria meningitidis carbohydrate fermentation patterns in Mueller-Hinton broth.
J Bacteriol. 1968 Aug;96(2):563 PMID: 4970654
Neisseria lactamicus sp. n., a lactose-fermenting species resembling Neisseria meningitidis.
Appl Microbiol. 1969 Jan;17(1):71-7 PMID: 4975454

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.21.6.1072-1074.1971

Published

1971-06-00

Pages

1072-4

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC377346

Subset

External Links

PubMed source DOI: 10.1128/am.21.6.1072-1074.1971 PMC Full Text

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Carbohydrate fermentation patterns of Neisseria meningitidis determined by a microtiter method.

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