Home LiteratureArticle Details
PMID: 8083991 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transduction of a human RNA sequence by poliovirus.

Journal of virology ·Vol. 68 ·No. 10 ·1994-10-00 ·Pages 6547-52

Charini WA, Todd S, Gutman GA, Semler BL

Abstract

Cells infected with poliovirus express a virally encoded polyprotein which undergoes self-mediated cleavage into structural and nonstructural viral proteins. Most of these cleavages are catalyzed by the 3C proteolytic domain of the polyprotein. Polyprotein synthesized in vitro from an RNA template containing a three-nucleotide insertion in 3C underwent proteolytic processing at all but one of the 3C-dependent cleavage sites. When transfected into HeLa cells, this RNA template displayed a lethal phenotype. We report here the isolation of two pseudorevertant progeny strains with restored protein-processing phenotypes, one of which appears to have arisen by transduction of a stretch of nucleotides from human 28S rRNA.

MeSH Terms
Amino Acid Sequence Base Sequence HeLa Cells Humans Methionine/metabolism Models, Genetic Molecular Sequence Data Mutagenesis, Site-Directed Phenotype Poliovirus/genetics,metabolism Protein Biosynthesis RNA, Ribosomal, 28S/biosynthesis RNA, Viral/metabolism Ribosomes/metabolism Templates, Genetic Transduction, Genetic Transfection Viral Proteins/biosynthesis
Chemicals
RNA, Ribosomal, 28S RNA, Viral Viral Proteins Methionine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Charini W A
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92717.
Todd S
Gutman G A
Semler B L
References (29)
29 references, click to expand
  1. Poliovirus RNA synthesis utilizes an RNP complex formed around the 5'-end of viral RNA.
    EMBO J. 1993 Sep;12(9):3587-98 PMID: 8253083
  2. trans rescue of a mutant poliovirus RNA polymerase function.
    J Virol. 1991 May;65(5):2655-65 PMID: 1850039
  3. The 5'-terminal sequence of potato leafroll virus RNA: evidence of recombination between virus and host RNA.
    J Gen Virol. 1991 Oct;72 ( Pt 10):2591-5 PMID: 1717645
  4. Role for the P4 amino acid residue in substrate utilization by the poliovirus 3CD proteinase.
    J Virol. 1991 Nov;65(11):6111-23 PMID: 1656088
  5. RNA recombination in animal and plant viruses.
    Microbiol Rev. 1992 Mar;56(1):61-79 PMID: 1579113
  6. Linker scanning mutagenesis of the internal ribosome entry site of poliovirus RNA.
    J Virol. 1992 Aug;66(8):5075-86 PMID: 1321289
  7. Poliovirus RNA recombination: mechanistic studies in the absence of selection.
    EMBO J. 1992 Aug;11(8):3135-45 PMID: 1379178
  8. Origin and replication of defective interfering particles.
    Curr Top Microbiol Immunol. 1981;93:151-207 PMID: 7026180
  9. Proteolytic processing of poliovirus polypeptides: antibodies to polypeptide P3-7c inhibit cleavage at glutamine-glycine pairs.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):3973-7 PMID: 6287457
  10. Intertypic recombination in poliovirus: genetic and biochemical studies.
    Virology. 1983 Jan 15;124(1):121-32 PMID: 6297149
  11. RNAs from two independently isolated defective interfering particles of Sindbis virus contain a cellular tRNA sequence at their 5' ends.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3279-83 PMID: 6304704
  12. Molecular cloning and complete sequence determination of RNA genome of human rhinovirus type 14.
    Proc Natl Acad Sci U S A. 1985 Feb;82(3):732-6 PMID: 2983312
  13. Poliovirus genome RNA hybridizes specifically to higher eukaryotic rRNAs.
    Nucleic Acids Res. 1985 Oct 11;13(19):6797-816 PMID: 2997728
  14. The mechanism of RNA recombination in poliovirus.
    Cell. 1986 Nov 7;47(3):433-43 PMID: 3021340
  15. Primary structure, gene organization and polypeptide expression of poliovirus RNA.
    Nature. 1981 Jun 18;291(5816):547-53 PMID: 6264310
  16. Variation among human 28S ribosomal RNA genes.
    Proc Natl Acad Sci U S A. 1985 Nov;82(22):7666-70 PMID: 3865188
  17. Genome of coxsackievirus B3.
    Virology. 1987 Jan;156(1):50-63 PMID: 3027968
  18. Site-specific mutagenesis of cDNA clones expressing a poliovirus proteinase.
    J Cell Biochem. 1987 Jan;33(1):39-51 PMID: 3029150
  19. Site-directed mutagenesis of proteinase 3C results in a poliovirus deficient in synthesis of viral RNA polymerase.
    J Virol. 1987 Jul;61(7):2162-70 PMID: 3035216
  20. An in vivo recombinant RNA capable of autocatalytic synthesis by Q beta replicase.
    Nature. 1988 Jun 2;333(6172):473-5 PMID: 2453805
  21. Protein 3CD is the major poliovirus proteinase responsible for cleavage of the P1 capsid precursor.
    Virology. 1988 Sep;166(1):265-70 PMID: 2842953
  22. Structural domains of the poliovirus polyprotein are major determinants for proteolytic cleavage at Gln-Gly pairs.
    J Biol Chem. 1988 Nov 25;263(33):17846-56 PMID: 2846581
  23. Cleavage of synthetic peptides by purified poliovirus 3C proteinase.
    J Biol Chem. 1989 Jun 15;264(17):9738-41 PMID: 2542331
  24. Increased viral pathogenicity after insertion of a 28S ribosomal RNA sequence into the haemagglutinin gene of an influenza virus.
    Nature. 1989 Jul 13;340(6229):156-7 PMID: 2544809
  25. Picornavirus protein processing--enzymes, substrates, and genetic regulation.
    Curr Top Microbiol Immunol. 1990;161:49-87 PMID: 2169385
  26. Poliovirus RNA replication.
    Curr Top Microbiol Immunol. 1990;161:89-119 PMID: 2169386
  27. Retroviral recombination and reverse transcription.
    Science. 1990 Nov 30;250(4985):1227-33 PMID: 1700865
  28. Viral cytopathogenicity correlated with integration of ubiquitin-coding sequences.
    Virology. 1991 Feb;180(2):602-16 PMID: 1846490
  29. Genetic recombination with Newcastle disease virus, polioviruses, and influenza.
    Cold Spring Harb Symp Quant Biol. 1962;27:303-9 PMID: 13954778
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.10.6547-6552.1994
Published
1994-10-00
Pages
6547-52
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237075
Subset
IM
Grants
NIAID NIH HHS · R01 AI022693 · United States
NIAID NIH HHS · AI22693 · United States
NIGMS NIH HHS · GM07134 · United States
External Links
PubMed source DOI: 10.1128/JVI.68.10.6547-6552.1994 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2