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PMID: 6304704 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

RNAs from two independently isolated defective interfering particles of Sindbis virus contain a cellular tRNA sequence at their 5' ends.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 11 ·1983-06-00 ·Pages 3279-83

Monroe SS, Schlesinger S

Abstract

Defective interfering (DI) particles are deletion mutants that interfere specifically with the replication of homologous standard virus. We have determined the 5'-terminal nucleotide sequences of two DI RNA populations by the following methods: (i) cloning of the cDNA from one of the DI RNA populations and sequencing a representative clone, and (ii) using both DI RNA populations as templates for preparing primer-directed cDNA transcripts and sequencing these transcripts. The 5' terminal sequences of the two DI RNA populations were not derived from standard Sindbis viral RNA but were almost identical to those of a cellular tRNAAsp.

MeSH Terms
Animals Base Sequence Chick Embryo Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes Fibroblasts Mutation Nucleic Acid Conformation Nucleic Acid Hybridization RNA, Transfer/genetics RNA, Viral/genetics Sindbis Virus/genetics
Chemicals
RNA, Viral DNA RNA, Transfer DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Monroe S S
Schlesinger S
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.11.3279
Published
1983-06-00
Pages
3279-83
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC394024
Subset
IM
Grants
NIAID NIH HHS · AI 11377 · United States
Databases
GENBANK
J02368, J02369, J02370, J02371, J02372, J02373, J02374, J02375, J02376, J02377, J02378, J02379, J02380, J02381, J02382, J02383, J02384, J02385
External Links
PubMed source DOI: 10.1073/pnas.80.11.3279 PMC Full Text View journal details
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