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PMID: 8045263 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Quality and position of the three lac operators of E. coli define efficiency of repression.

The EMBO journal ·Vol. 13 ·No. 14 ·1994-07-15 ·Pages 3348-55

Oehler S, Amouyal M, Kolkhof P, von Wilcken-Bergmann B, Müller-Hill B

Abstract

Repression of the lac promoter may be achieved in two different ways: either by interference with the action of RNA polymerase or by interference with CAP activation. We investigated cooperative repression of the Escherichia coli lac operon by systematic conversion of its three natural operators (O1, O2 and O3) on the chromosome. We find that cooperative repression by tetrameric Lac repressor increases with both quality and proximity of the interacting operators. A short distance of 92 bp allows effective repression by two very weak operators (O3, O3). The cooperativity of lac operators is discussed in terms of a local increase of repressor concentration. This increase in concentration depends on flexible DNA which allows loop formation.

MeSH Terms
Base Sequence DNA, Bacterial/genetics Enzyme Repression Escherichia coli/genetics Gene Expression Regulation, Bacterial Lac Operon/genetics Models, Genetic Molecular Sequence Data Nucleic Acid Conformation Operator Regions, Genetic/genetics Protein Binding Repressor Proteins/metabolism Structure-Activity Relationship
Chemicals
DNA, Bacterial Repressor Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Oehler S
Institut für Genetik der Universität zu Köln, Germany.
Amouyal M
Kolkhof P
von Wilcken-Bergmann B
Müller-Hill B
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44 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-07-15
Pages
3348-55
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395232
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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