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PMID: 3059350 Published · ppublish English Journal Article

Interaction of spatially separated protein-DNA complexes for control of gene expression: operator conversions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 24 ·1988-12-00 ·Pages 9683-7

Haber R, Adhya S

Abstract

Two operators, spatially separated from each other and from the promoters, repress the gal operon when bound to Gal repressor. Conversion of either gal operator to a lac operator results in derepression, although both Gal and Lac repressors are present, suggesting that mere occupation of operator sites is not sufficient to cause repression. Conversion of both operators to lac operators restores normal repression in the presence of Lac repressor protein. We propose that normal repression requires interaction between operator-bound like repressor molecules; this generates a DNA loop, which is part of a higher order structure. RNA polymerase and cyclic AMP receptor protein are present in this complex but unable to initiate transcription because of the higher order structure. Such higher order DNA-multiprotein complexes could occur in a variety of genetic regulatory systems that are controlled from distal sites by regulatory proteins.

MeSH Terms
Base Sequence DNA-Directed RNA Polymerases/analysis Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation Genes Genes, Bacterial Macromolecular Substances Multiprotein Complexes Nucleic Acid Conformation Operator Regions, Genetic Operon Plasmids Receptors, Cyclic AMP/analysis Repressor Proteins/metabolism
Chemicals
Escherichia coli Proteins Galactose repressor proteins Macromolecular Substances Multiprotein Complexes Receptors, Cyclic AMP Repressor Proteins DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Haber R
Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Adhya S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.24.9683
Published
1988-12-00
Pages
9683-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282836
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.24.9683 PMC Full Text View journal details
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