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PMID: 7962101 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Modulation of cell surface fibronectin assembly sites by lysophosphatidic acid.

The Journal of cell biology ·Vol. 127 ·No. 5 ·1994-12-00 ·Pages 1447-59

Zhang Q, Checovich WJ, Peters DM, Albrecht RM, Mosher DF

Abstract

Lysophosphatidic acid is a product of activated platelets and has diverse actions on cells. We have characterized the effect of lysophosphatidic acid on cell-mediated binding and assembly of fibronectin, an extracellular matrix protein. Serum made from whole blood, but neither platelet-poor plasma nor serum made from platelet-poor plasma, caused enhanced binding of fibronectin to cultured fibroblastic cells. The ability of whole blood serum to enhance binding of fibronectin was abolished by phospholipase B. These results indicate that lysophosphatidic acid derived from platelets is the principal component in whole blood serum that is active in the fibronectin binding assay. 1-oleoyl lysophosphatidic acid, 20-200 nM, was as active as 0.1-0.2% whole blood serum. The stimulatory effect of lysophosphatidic acid on the binding of fibronectin or the amino-terminal 70-kD fragment of fibronectin was rapid, sustained, and lost upon removal of lysophosphatidic acid. The stimulatory effect on binding could not be duplicated by bradykinin, platelet-activating factor, bombesin, or a peptide agonist of the thrombin receptor. Enhanced binding of the 70-kD fragment was due to increases in both the number and affinity of binding sites. Enhanced binding and assembly of fibronectin correlated with changes in cell shape and actin-containing cytoskeleton. The binding sites for fibronectin on lysophosphatidic acid-stimulated cells, as assessed by fluorescence, video, and scanning electron microscopy, were on areas of cell membrane containing numerous filopodia that extended between cells or between cells and substratum. These observations suggest that lysophosphatidic acid functions as a powerful and specific modulator of cell shape and early matrix assembly during wound healing.

MeSH Terms
Actin Cytoskeleton/drug effects,metabolism,ultrastructure Binding Sites Blood Bradykinin/pharmacology Cell Membrane/drug effects,metabolism Cell Size/drug effects Dose-Response Relationship, Drug Fibronectins/metabolism Humans Kinetics Lysophospholipids/pharmacology Microscopy, Electron, Scanning Peptide Fragments/pharmacology Platelet Activating Factor/pharmacology Receptors, Thrombin Tumor Cells, Cultured
Chemicals
Fibronectins Lysophospholipids Peptide Fragments Platelet Activating Factor Receptors, Thrombin lysophosphatidic acid Bradykinin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zhang Q
Department of Medicine, University of Wisconsin-Madison 53706.
Checovich W J
Peters D M
Albrecht R M
Mosher D F
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.127.5.1447
Published
1994-12-00
Pages
1447-59
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120255
Subset
IM
Grants
NIGMS NIH HHS · GM-47221 · United States
NHLBI NIH HHS · HL-21644 · United States
External Links
PubMed source DOI: 10.1083/jcb.127.5.1447 PMC Full Text View journal details
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