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PMID: 3054513 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transforming growth factor beta increases cell surface binding and assembly of exogenous (plasma) fibronectin by normal human fibroblasts.

Molecular and cellular biology ·Vol. 8 ·No. 10 ·1988-10-00 ·Pages 4234-42

Allen-Hoffmann BL, Crankshaw CL, Mosher DF

Abstract

Transforming growth factor beta (TGF-beta) enhances the cell surface binding of 125I-fibronectin by cultured human fibroblasts. The effect of TGF-beta on cell surface binding was maximal after 2 h of exposure to TFG-beta and did not require epidermal growth factor or protein synthesis. The enhancement was dose dependent and was found with the 125I-labeled 70-kilodalton amino-terminal fragment of fibronectin as well as with 125I-fibronectin. Treatment of cultures with TGF-beta for 6 h resulted in a threefold increase in the estimated number of fibronectin binding sites. The increase in number of binding sites was accompanied by an increased accumulation of labeled fibronectin in detergent-insoluble extracellular matrix. The effect of TGF-beta was biphasic; after 6 h of exposure, less labeled fibronectin bound to treated cultures than to control cultures. Exposure of cells to TGF-beta for greater than 6 h caused a two- to threefold increase in the accumulation of cellular fibronectin in culture medium as detected by a quantitative enzyme-linked immunosorbent assay. The second phase of the biphasic effect and the increase in soluble cellular fibronectin were blocked by cycloheximide. Immunofluorescence staining of fibroblast cultures with antifibronectin revealed that TGF-beta caused a striking increase in fibronectin fibrils. The 70-kilodalton amino-terminal fragment of fibronectin, which blocks incorporation of fibronectin into extracellular matrix, blocked anchorage-independent growth of NRK-49F cells in the presence of epidermal growth factor. Our results show that an increase in the binding and rate of assembly of exogenous fibronectin is an early event preceding the increase in expression of extracellular matrix proteins. Such an early increase in cell surface binding of exogenous fibronectin may be a mechanism whereby TGF-beta can modify extracellular matrix characteristics rapidly after tissue injury or during embryonic morphogenesis.

MeSH Terms
Cycloheximide/pharmacology Extracellular Matrix/physiology Fibronectins/metabolism Fluorescent Antibody Technique Humans In Vitro Techniques Macromolecular Substances Protein Binding/drug effects Transforming Growth Factors/pharmacology
Chemicals
Fibronectins Macromolecular Substances Transforming Growth Factors Cycloheximide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Allen-Hoffmann B L
Department of Pathology, University of Wisconsin, Madison 53706.
Crankshaw C L
Mosher D F
References (43)
43 references, click to expand
  1. Exogenous gangliosides enhance the interaction of fibronectin with ganglioside-deficient cells.
    Exp Cell Res. 1983 Feb;143(2):295-302 PMID: 6832218
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. Growth factors from murine sarcoma virus-transformed cells.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):4001-5 PMID: 211512
  4. Biosynthesis and processing of fibronectin in NIL.8 hamster cells.
    J Biol Chem. 1979 Dec 10;254(23):12050-5 PMID: 500694
  5. Distribution of fetal bovine serum fibronectin and endogenous rat cell fibronectin in extracellular matrix.
    J Cell Biol. 1979 Oct;83(1):255-9 PMID: 389940
  6. Sequential appearance of fibronectin and collagen in experimental granulation tissue.
    Lab Invest. 1980 Jul;43(1):47-51 PMID: 6993786
  7. Kirsten murine sarcoma virus transformed cell lines and a spontaneously transformed rat cell-line produce transforming factors.
    J Cell Physiol. 1980 Oct;105(1):163-80 PMID: 6253509
  8. Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule.
    Cell. 1981 Oct;26(2 Pt 2):259-67 PMID: 6174240
  9. The cell attachment domain of fibronectin. Determination of the primary structure.
    J Biol Chem. 1982 Aug 25;257(16):9593-7 PMID: 7050098
  10. Serum contains a platelet-derived transforming growth factor.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5312-6 PMID: 6957865
  11. Polypeptide transforming growth factors isolated from bovine sources and used for wound healing in vivo.
    Science. 1983 Mar 18;219(4590):1329-31 PMID: 6572416
  12. Binding of plasma fibronectin to cell layers of human skin fibroblasts.
    J Cell Biol. 1983 Aug;97(2):466-72 PMID: 6309861
  13. Transforming growth factor-beta controls receptor levels for epidermal growth factor in NRK fibroblasts.
    Cell. 1984 Jan;36(1):35-41 PMID: 6319010
  14. The role of intermolecular disulfide bonding in deposition of GP140 in the extracellular matrix.
    J Cell Biol. 1984 Jul;99(1 Pt 1):105-14 PMID: 6736123
  15. Dualistic nature of adhesive protein function: fibronectin and its biologically active peptide fragments can autoinhibit fibronectin function.
    J Cell Biol. 1984 Jul;99(1 Pt 1):29-36 PMID: 6736130
  16. Mechanism of formation of disulfide-bonded multimers of plasma fibronectin in cell layers of cultured human fibroblasts.
    J Biol Chem. 1984 Oct 10;259(19):12210-5 PMID: 6480605
  17. Variants of the cell recognition site of fibronectin that retain attachment-promoting activity.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):5985-8 PMID: 6237366
  18. Biologically active synthetic peptides as probes of embryonic development: a competitive peptide inhibitor of fibronectin function inhibits gastrulation in amphibian embryos and neural crest cell migration in avian embryos.
    J Cell Biol. 1984 Nov;99(5):1822-30 PMID: 6490722
  19. Cleavage of a 135 kD cell surface glycoprotein correlates with loss of fibroblast adhesion to fibronectin.
    Exp Cell Res. 1985 Jan;156(1):182-90 PMID: 2981173
  20. Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptor.
    Cell. 1985 Jan;40(1):191-8 PMID: 3155652
  21. Interaction of the 70,000-mol-wt amino-terminal fragment of fibronectin with the matrix-assembly receptor of fibroblasts.
    J Cell Biol. 1985 Feb;100(2):364-74 PMID: 3155749
  22. A monoclonal antibody identifies a glycoprotein complex involved in cell-substratum adhesion.
    Exp Cell Res. 1985 Mar;157(1):218-26 PMID: 2578969
  23. Fluorescent gangliosides as probes for the retention and organization of fibronectin by ganglioside-deficient mouse cells.
    J Cell Biol. 1985 Mar;100(3):721-6 PMID: 3882721
  24. Fibronectin and wound healing.
    J Cell Biochem. 1984;26(2):107-16 PMID: 6084665
  25. Development of cell surface linkage complexes in cultured fibroblasts.
    J Cell Biol. 1985 Apr;100(4):1103-14 PMID: 3884631
  26. Distribution of the cell substratum attachment (CSAT) antigen on myogenic and fibroblastic cells in culture.
    J Cell Biol. 1985 May;100(5):1528-39 PMID: 3921554
  27. Incorporation of cellular and plasma fibronectins into smooth muscle cell extracellular matrix in vitro.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2746-50 PMID: 3887410
  28. Selective inhibition of fibronectin-mediated cell adhesion by monoclonal antibodies to a cell-surface glycoprotein.
    Science. 1985 Jun 21;228(4706):1448-51 PMID: 4012302
  29. Transforming growth factor-beta stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix.
    J Biol Chem. 1986 Mar 25;261(9):4337-45 PMID: 3456347
  30. Localization of the ganglioside-binding site of fibronectin.
    J Biol Chem. 1986 Apr 15;261(11):5209-14 PMID: 3957921
  31. Transforming growth factor type beta: rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4167-71 PMID: 2424019
  32. Localization of cell surface sites involved in fibronectin fibrillogenesis.
    J Cell Biol. 1987 Jan;104(1):121-30 PMID: 2947903
  33. Fibronectin's cell-adhesive domain and an amino-terminal matrix assembly domain participate in its assembly into fibroblast pericellular matrix.
    J Biol Chem. 1987 Mar 5;262(7):2957-67 PMID: 3818629
  34. Induction of fibronectin matrix assembly in human fibrosarcoma cells by dexamethasone.
    J Cell Biol. 1987 Mar;104(3):601-10 PMID: 2950120
  35. Transforming growth factor-beta increases steady state levels of type I procollagen and fibronectin messenger RNAs posttranscriptionally in cultured human dermal fibroblasts.
    J Clin Invest. 1987 Apr;79(4):1285-8 PMID: 3470308
  36. Regulation of fibronectin and type I collagen mRNA levels by transforming growth factor-beta.
    J Biol Chem. 1987 May 15;262(14):6443-6 PMID: 3471760
  37. Fibronectin-associated transforming growth factor.
    J Cell Physiol. 1987 May;131(2):184-9 PMID: 3495542
  38. Beta-type transforming growth factor specifies organizational behavior in vascular smooth muscle cell cultures.
    J Cell Biol. 1987 Jul;105(1):465-71 PMID: 3475277
  39. Accelerated healing of incisional wounds in rats induced by transforming growth factor-beta.
    Science. 1987 Sep 11;237(4820):1333-6 PMID: 2442813
  40. Matrix assembly sites for exogenous fibronectin are decreased on human fibroblasts after treatment with agents which increase intracellular cAMP.
    J Biol Chem. 1987 Oct 15;262(29):14361-5 PMID: 2821002
  41. Cell adhesion protein receptors as targets for transforming growth factor-beta action.
    Cell. 1987 Oct 23;51(2):189-97 PMID: 3499229
  42. Transforming growth factor beta stimulates the expression of fibronectin and of both subunits of the human fibronectin receptor by cultured human lung fibroblasts.
    J Biol Chem. 1988 Apr 5;263(10):4586-92 PMID: 2965146
  43. Transforming growth factor-beta in human platelets. Identification of a major storage site, purification, and characterization.
    J Biol Chem. 1983 Jun 10;258(11):7155-60 PMID: 6602130
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.10.4234-4242.1988
Published
1988-10-00
Pages
4234-42
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365495
Subset
IM
Grants
NHLBI NIH HHS · HL 21644 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.10.4234-4242.1988 PMC Full Text View journal details
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