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PMID: 8489494 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The bioactive phospholipid lysophosphatidic acid is released from activated platelets.

The Biochemical journal ·Vol. 291 ( Pt 3) ·1993-05-01 ·Pages 677-80

Eichholtz T, Jalink K, Fahrenfort I, Moolenaar WH

Abstract

Lysophosphatidic acid (LPA) is a water-soluble phospholipid with hormone-like and growth-factor-like activities. LPA activates a putative G-protein-coupled receptor in responsive cells, but the natural source of exogenous LPA is unknown. Here we show that LPA is present in mammalian serum in an active form (bound to albumin) at concentrations of 1-5 microM, but is not detectable in platelet-poor plasma, suggesting that LPA is produced during blood clotting. We find that thrombin activation of platelets prelabelled with [32P]Pi results in the rapid release of newly formed [32P]LPA into the extracellular environment. We conclude that LPA is a novel platelet-derived lipid mediator that may play a role in inflammatory and proliferative responses to injury.

MeSH Terms
Blood Platelets/metabolism Humans Lysophospholipids/blood Phosphates/metabolism Platelet Activation/drug effects Thrombin/pharmacology
Chemicals
Lysophospholipids Phosphates Thrombin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Eichholtz T
Division of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam.
Jalink K
Fahrenfort I
Moolenaar W H
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34 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2910677
Published
1993-05-01
Pages
677-80
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1132420
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2910677 PMC Full Text View journal details
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