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PMID: 7927711 Published · ppublish English Journal Article

Nucleotide sequence of the Streptococcus gordonii PK488 coaggregation adhesin gene, scaA, and ATP-binding cassette.

Infection and immunity ·Vol. 62 ·No. 10 ·1994-10-00 ·Pages 4469-80

Kolenbrander PE, Andersen RN, Ganeshkumar N

Abstract

Human oral viridans group streptococci that coaggregate with Actinomyces naeslundii PK606 express surface proteins related to ScaA, the coaggregation-mediating adhesin of Streptococcus gordonii PK488 (R. N. Andersen, N. Ganeshkumar, and P. E. Kolenbrander, Infect. Immun. 61:981-987, 1993). The nucleotide sequence of the 6,125-bp EcoRI insert of pRA1, containing scaA, the gene encoding ScaA, was determined. Six open reading frames (ORFs) were identified. The orientation of four ORFs, two upstream (ORF 1 and ORF 2) and one downstream (ORF 4) of scaA (ORF 3), indicated transcription in one direction, whereas ORF 5 and ORF 6 were transcribed divergently. Computer analysis of the deduced amino acid sequences identified a consensus binding site for ATP (GxxGxGKS) in the putative 28,054-Da protein encoded by ORF 1. ORF 2 potentially encoded a hydrophobic protein of 29,705 Da with six potential membrane-spanning regions. ScaA was 310 amino acids, 34,787 Da, and contained the lipoprotein consensus sequence LxxC, also reported for the ScaA-related proteins SsaB, FimA, and PsaA from Streptococcus sanguis 12, Streptococcus parasanguis FW213, and Streptococcus pneumoniae R36A, respectively. ORF 4 potentially encoded a 163-amino-acid protein of 17,912 Da, which was nearly identical to the downstream adjacent gene products of ssaB, fimA, and psaA. No significant homology with other proteins was found with the putative ORF 5 gene product, a 229-amino-acid protein of 25,107 Da. ORF 6 was incomplete and encoded a protein larger than 564 amino acids. This putative protein had a consensus Zn2+ binding motif, HExxH, found among bacterial thermolysins and mammalian neutral endopeptidases and was 40% identical to a homologous 210-amino-acid region of human enkephalinase. The genetic organization of ORFs 1, 2, and 3 was similar to those of the bacterial periplasmic-binding protein-dependent transport systems of gram-negative bacteria and binding-lipoprotein-dependent transport systems of gram-positive bacteria, and these genes appeared to encode ABC (ATP-binding cassette) proteins. This report describes a cell-to-cell adherence function associated with an ATP-binding cassette.

Related Genes
scaA
MeSH Terms
Adenosine Triphosphate/metabolism Amino Acid Sequence Bacterial Adhesion Bacterial Proteins/genetics Base Sequence Carrier Proteins/genetics DNA, Bacterial/chemistry Genes, Bacterial Membrane Proteins/analysis Molecular Sequence Data Open Reading Frames Restriction Mapping Streptococcus/genetics Zinc/metabolism
Chemicals
Bacterial Proteins Carrier Proteins DNA, Bacterial Membrane Proteins ScaA protein, Streptococcus gordonii Adenosine Triphosphate Zinc
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kolenbrander P E
Laboratory of Microbial Ecology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
Andersen R N
Ganeshkumar N
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.62.10.4469-4480.1994
Published
1994-10-00
Pages
4469-80
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC303132
Subset
IM
Databases
GENBANK
L11577
External Links
PubMed source DOI: 10.1128/iai.62.10.4469-4480.1994 PMC Full Text View journal details
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