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PMID: 8225574 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of the surface component of Streptococcus defectivus that mediates extracellular matrix adherence.

Infection and immunity ·Vol. 61 ·No. 12 ·1993-12-00 ·Pages 4994-5000

Tart RC, van de Rijn I

Abstract

Bacterial attachment to host tissue is considered to be a crucial primary step in pathogen infection. Previous studies have shown that Streptococcus defectivus adheres specifically to cell-secreted extracellular matrix (ECM). Though generally not exposed in vivo, this host tissue is exposed at endothelial cell junctions and sites of tissue injury. In this report, we identify a ca. 200-kDa surface protein of S. defectivus involved in ECM adherence. Nitrous acid-derived mutant strains that were unable to bind ECM and which failed to adsorb adhesin-specific antibody from polyclonal inhibitory sera were isolated. A surface protein (ca. 200 kDa) was absent from ECM-nonadherent mutants, indicating its involvement in ECM attachment. Additionally, affinity-purified antibody to the ca. 200-kDa protein inhibited whole-cell S. defectivus ECM attachment, whereas antibody to the same region of the nonadherent mutant cell wall-associated protein profile did not. Furthermore, solubilized cell wall-associated protein extracts of parent but not mutant strains bound ECM, confirming the significance of this protein in ECM adherence. Therefore, we propose that the ca. 200-kDa protein is the major S. defectivus surface component that mediates the ECM attachment of these organisms.

MeSH Terms
Adhesins, Bacterial Animals Antigens, Bacterial/chemistry,genetics,physiology Bacterial Adhesion/genetics,physiology Bacterial Proteins/chemistry,immunology,physiology Cells, Cultured Cricetinae Extracellular Matrix/physiology In Vitro Techniques Molecular Weight Mutation Streptococcus/genetics,pathogenicity,physiology
Chemicals
Adhesins, Bacterial Antigens, Bacterial Bacterial Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tart R C
Wake Forest University Medical Center, Winston-Salem, North Carolina 27157-1064.
van de Rijn I
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.12.4994-5000.1993
Published
1993-12-00
Pages
4994-5000
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC281274
Subset
IM
Grants
NHLBI NIH HHS · HL30069 · United States
NIAID NIH HHS · T32-AI-07401 · United States
External Links
PubMed source DOI: 10.1128/iai.61.12.4994-5000.1993 PMC Full Text View journal details
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