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PMID: 2332509 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interaction between Borrelia burgdorferi and endothelium in vitro.

The Journal of clinical investigation ·Vol. 85 ·No. 5 ·1990-05-00 ·Pages 1637-47

Szczepanski A, Furie MB, Benach JL, Lane BP, Fleit HB

Abstract

During the pathogenesis of Lyme disease, Borrelia burgdorferi spreads hematogenously from the site of a tick bite to several tissues throughout the body. The specific mechanism of spirochete emigration is presently unknown. Using cultured human umbilical vein endothelial cells, we found that Borrelia burgdorferi bound to the endothelial cells and to the subendothelial matrix. Low passage isolates adhered 22-30-fold greater than a strain maintained in culture continuously. Spirochete binding to subendothelial matrix was inhibited 48-63% by pretreatment of the matrix with anti-fibronectin antiserum. Spirochete migration across endothelial monolayers cultured on amniotic membrane was increased when the monolayers were damaged by chemical or physical means. Electron microscopic examination of spirochete-endothelial interactions demonstrated the presence of spirochetes in the intercellular junctions between endothelial cells as well as beneath the monolayers. Scanning electron microscopy identified a mechanism of transendothelial migration whereby spirochetes pass between cells into the amniotic membrane at areas where subendothelium is exposed.

MeSH Terms
Amnion/cytology,microbiology,ultrastructure Bacterial Adhesion Borrelia/isolation & purification Borrelia burgdorferi Group/isolation & purification,physiology,ultrastructure Cells, Cultured Endothelium, Vascular/microbiology,ultrastructure Female Humans Microscopy, Electron Microscopy, Electron, Scanning Pregnancy Umbilical Veins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Szczepanski A
Department of Pathology, State University of New York, Stony Brook 11794.
Furie M B
Benach J L
Lane B P
Fleit H B
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI114615
Published
1990-05-00
Pages
1637-47
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC296616
Subset
IM
Grants
PHS HHS · 27044 · United States
External Links
PubMed source DOI: 10.1172/JCI114615 PMC Full Text View journal details
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