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PMID: 8454362 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of a salivary agglutinin-binding domain within cell surface adhesin P1 of Streptococcus mutans.

Infection and immunity ·Vol. 61 ·No. 4 ·1993-04-00 ·Pages 1547-52

Crowley PJ, Brady LJ, Piacentini DA, Bleiweis AS

Abstract

DNA encoding the alanine-rich region (A-region) of the cell surface adhesin, P1, from Streptococcus mutans was subcloned and expressed as a fusion protein with the maltose-binding protein (MBP) of Escherichia coli. The A-region fusion protein was shown to competitively inhibit both adherence of S. mutans to salivary agglutinin-coated hydroxyapatite and fluid-phase agglutinin-mediated aggregation of this organism. MBP alone or an MBP-paramyosin fusion protein was not inhibitory. Proteolytic cleavage of the fusion protein into its component moieties, MBP and A-region, resulted in breakdown of the A-region into three main fragments. Western immunoblot analysis of calcium-dependent agglutinin binding to this preparation revealed binding specificity for a 28-kDa fragment. Thus, the A-region of P1 is an important domain which interacts directly with salivary agglutinin, and this interaction interferes with both the aggregation and the adherence mechanisms in vitro.

Related Genes
spaP
MeSH Terms
Adhesins, Bacterial Agglutinins/metabolism Amino Acid Sequence Bacterial Adhesion Bacterial Proteins/immunology,metabolism Base Sequence Cloning, Molecular DNA, Bacterial/genetics Genes, Bacterial Humans In Vitro Techniques Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Protein Binding Recombinant Fusion Proteins Salivary Proteins and Peptides/metabolism Streptococcus mutans/cytology,pathogenicity
Chemicals
Adhesins, Bacterial Agglutinins Bacterial Proteins DNA, Bacterial Oligodeoxyribonucleotides Recombinant Fusion Proteins Salivary Proteins and Peptides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Crowley P J
Department of Oral Biology, University of Florida, Gainesville 32610.
Brady L J
Piacentini D A
Bleiweis A S
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33 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.4.1547-1552.1993
Published
1993-04-00
Pages
1547-52
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC281399
Subset
IM
Grants
NIDCR NIH HHS · R37-DE-08007 · United States
External Links
PubMed source DOI: 10.1128/iai.61.4.1547-1552.1993 PMC Full Text View journal details
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