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PMID: 7691754 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A protein fragment of streptococcal cell surface antigen I/II which prevents adhesion of Streptococcus mutans.

Infection and immunity ·Vol. 61 ·No. 11 ·1993-11-00 ·Pages 4590-8

Munro GH, Evans P, Todryk S, Buckett P, Kelly CG, Lehner T

Abstract

Attachment of Streptococcus mutans to the tooth surface involves a cell surface protein with an M(r) of 185,000, termed streptococcal antigen (SA) I/II. Four overlapping fragments of the gene encoding SA I/II were amplified by polymerase chain reaction, cloned, and expressed in Escherichia coli. The recombinant polypeptides were assayed for adhesion-binding activity to salivary receptors and for recognition by a panel of monoclonal antibodies (MAbs) raised against SA I/II. Two of the MAbs which are known to prevent colonization of S. mutans in vivo bound the recombinant polypeptide comprising residues 816 to 1161. In vitro adhesion of S. mutans to saliva-coated hydroxyapatite beads was also inhibited specifically by a polypeptide (residues 816 to 1213) encompassing the same region. The evidence from the MAbs preventing colonization of S. mutans and the adherence inhibition assay suggests that an adhesion-binding activity resides within the portion of SA I/II comprising residues 816 to 1213, which is highly conserved among oral streptococcal species.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antigens, Bacterial/metabolism Bacterial Adhesion Bacterial Proteins/chemistry,immunology,metabolism Base Sequence Binding Sites Epitopes Membrane Glycoproteins Molecular Sequence Data Peptide Fragments/metabolism Rabbits Recombinant Proteins/isolation & purification,pharmacology Streptococcus mutans/immunology,physiology
Chemicals
Antibodies, Monoclonal Antigens, Bacterial Bacterial Proteins Epitopes Membrane Glycoproteins Peptide Fragments Recombinant Proteins S-layer proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Munro G H
Department of Immunology, United Medical School, Guy's Hospital, London, United Kingdom.
Evans P
Todryk S
Buckett P
Kelly C G
Lehner T
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.11.4590-4598.1993
Published
1993-11-00
Pages
4590-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC281209
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.61.11.4590-4598.1993 PMC Full Text View journal details
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