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PMID: 7906225 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Involvement of c-Jun in the control of glucocorticoid receptor transcriptional activity during development of chicken retinal tissue.

The EMBO journal ·Vol. 13 ·No. 3 ·1994-02-01 ·Pages 646-54

Berko-Flint Y, Levkowitz G, Vardimon L

Abstract

The ability of the glucocorticoid receptor (GR) to induce gene expression in embryonic chicken retinal tissue increases dramatically during development, although the quantity of the receptor molecules does not change greatly with age. This study examines the possible involvement of c-Jun in the developmental control of GR activity. Expression of c-Jun in retinal tissue was high at early embryonic ages and declined during development. Elevation of c-Jun expression in retina of mid-developmental ages by treatment with 12-O-tetradecanoyl-phorbol-13-acetate (TPA), or by introduction of a c-Jun expression vector, caused a pronounced decline in the inducibility of the endogenous glutamine synthetase gene and the transiently transfected CAT constructs p delta G46TCO and pGS2.1CAT, that are controlled by a minimal consensus glucocorticoid response element (GRE) promoter and the glutamine synthetase promoter, respectively. The effect of c-Jun was dose dependent and could be reversed by overexpression of GR. C-Jun-evoked repression of GR activity could be relieved by overexpression of Jun D. Overexpression of Jun D could also elevate the responsiveness of early embryonic retina to glucocorticoids and cause a 5-fold increase in p delta G46TCO induction. The effect of Jun D could be reversed by overexpression of c-Jun. Expression of c-Jun might therefore be important for repression of GR activity at early embryonic ages.

MeSH Terms
Animals Chick Embryo Chloramphenicol O-Acetyltransferase/genetics,metabolism Culture Techniques Enzyme Induction Gene Expression Regulation Glutamate-Ammonia Ligase/biosynthesis,genetics Proto-Oncogene Proteins c-jun/metabolism Receptors, Glucocorticoid/biosynthesis,genetics Retina/embryology,metabolism Tetradecanoylphorbol Acetate/pharmacology Transcription, Genetic Transfection
Chemicals
Proto-Oncogene Proteins c-jun Receptors, Glucocorticoid Chloramphenicol O-Acetyltransferase Glutamate-Ammonia Ligase Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Berko-Flint Y
Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Israel.
Levkowitz G
Vardimon L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-02-01
Pages
646-54
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394855
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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