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PMID: 8093326 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Involvement of a C/EBP-like protein in the acquisition of responsiveness to glucocorticoid hormones during chick neural retina development.

Molecular and cellular biology ·Vol. 13 ·No. 1 ·1993-01-00 ·Pages 331-40

Ben-Or S, Okret S

Abstract

The glucocorticoid receptor in chicken embryonic neural retina is expressed early in ontogeny, yet the tissue's response to the glucocorticoid hormone, i.e., induction of glutamine synthetase (GS), develops later, only during week 2 of ontogeny. Transient transfection of embryonic day 7 (E7) retinal cells, which are nonresponsive to glucocorticoids, with chimeric plasmids containing the chloramphenicol acetyltransferase reporter gene under the control of glucocorticoid-responsive promoters demonstrated that GR in E7 cells is a functional transactivating factor. We show that the limiting transcription factor that controls the developmental acquisition of responsiveness to glucocorticoids is similar to a CCAAT enhancer-binding protein (C/EBP). This protein recognizes a sequence in the promoter of the chick GS gene, which is required for eliciting the glucocorticoid response. Retinal C/EBP-like protein was not detected in the glucocorticoid-nonresponsive (E7) proliferating glioblasts but was found to be present in the glucocorticoid-responsive (E12) postmitotic cells. Premature expression of C/EBP in the nonresponsive E7 cells by transfection was shown to enhance the developmental acquisition of responsiveness to the glucocorticoid hormone, as deduced from the level of GS inducibility.

MeSH Terms
Animals Base Sequence CCAAT-Enhancer-Binding Proteins Chick Embryo DNA-Binding Proteins/metabolism,physiology Gene Expression/drug effects Glucocorticoids/pharmacology Glutamate-Ammonia Ligase/genetics Host Cell Factor C1 Molecular Sequence Data Nuclear Proteins/physiology Octamer Transcription Factor-1 Oligodeoxyribonucleotides/chemistry RNA, Messenger/genetics Receptors, Glucocorticoid/physiology Retina/embryology Transcription Factors/metabolism,physiology Transfection
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins Glucocorticoids Host Cell Factor C1 Nuclear Proteins Octamer Transcription Factor-1 Oligodeoxyribonucleotides RNA, Messenger Receptors, Glucocorticoid Transcription Factors Glutamate-Ammonia Ligase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ben-Or S
Department of Physiology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
Okret S
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.1.331-340.1993
Published
1993-01-00
Pages
331-40
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358912
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.13.1.331-340.1993 PMC Full Text View journal details
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